BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0280b.3
(577 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI420177 38 0.004
BP034655 35 0.025
TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%) 34 0.074
TC10919 similar to UP|SRS1_ORYSA (P83649) Salt-stress root prote... 32 0.28
BP042644 32 0.28
TC17290 similar to UP|Q8H4Y5 (Q8H4Y5) OJ1136_A10.4 protein, part... 32 0.37
TC14217 32 0.37
TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB... 32 0.37
TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetyla... 30 1.1
AV408367 30 1.1
TC19394 similar to UP|NSR1_YEAST (P27476) Nuclear localization s... 30 1.4
BP057817 29 1.8
TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associ... 29 1.8
BP030312 29 1.8
AV410494 29 1.8
TC10270 homologue to UP|Q8VXT0 (Q8VXT0) Translation initiation f... 29 2.4
TC8736 similar to PIR|T06396|T06396 isoprenylated protein - soyb... 29 2.4
BP042908 29 2.4
AV780534 28 3.1
BP038767 28 3.1
>BI420177
Length = 444
Score = 38.1 bits (87), Expect = 0.004
Identities = 30/133 (22%), Positives = 48/133 (35%), Gaps = 14/133 (10%)
Frame = +3
Query: 41 VSSSHVLLEDVTSDEEEEPFHDAPANINEEETL----------MGSDRDV----VPDAEA 86
++S LL +EEEE +AP T +G+D + VP
Sbjct: 15 LASMDSLLATYKDEEEEEDEEEAPPTTTTTSTAADGGDGGGVAVGADTEPIPIDVPPVVT 194
Query: 87 SAPQDTNVSQENPIPENPDVEDVIAEETVSTETPSSSKKGDKGSVSSKETHSDEPIQVLV 146
S P DT +N PD D ++ E +++ S K + + +P+ L
Sbjct: 195 STPSDTQTEPQNDGRSEPDPSDDLSSEEAASDGSKKSPKRELDEEQQPASKKQKPLSSLS 374
Query: 147 QNNSDGDDSDDDD 159
N D + D
Sbjct: 375 AANQDSPALPEGD 413
>BP034655
Length = 517
Score = 35.4 bits (80), Expect = 0.025
Identities = 19/50 (38%), Positives = 24/50 (48%)
Frame = +2
Query: 176 RKRRTTASEAPSVPQKKTKPSPTTPKSKKKDLKGKGKATAEKSMSEKKKK 225
R+ T P + + SPTT K KKK K K K +K +KKKK
Sbjct: 71 RRNFTATKSPPHISPPQILDSPTTMKRKKKKKKKKKKKKKKKKKKKKKKK 220
>TC11317 similar to UP|Q40363 (Q40363) NuM1 protein, partial (12%)
Length = 547
Score = 33.9 bits (76), Expect = 0.074
Identities = 26/87 (29%), Positives = 41/87 (46%), Gaps = 12/87 (13%)
Frame = +1
Query: 85 EASAPQDTNVSQENPIPENPDVEDVIAEETVSTETPSSSKKGDKGSVSSKETHSDE---- 140
E+S+ D++ E P P+ ++ + T TP+ K S SS E SDE
Sbjct: 277 ESSSDDDSSSEDEKPAPKVAPLKTSVKNGT----TPAKKAKPAPSSSSSDEDSSDEEEEV 444
Query: 141 ----PIQVLV----QNNSDGDDSDDDD 159
P +V+V +++SD D S D+D
Sbjct: 445 IAKKPTKVVVPKKEESSSDEDSSSDED 525
>TC10919 similar to UP|SRS1_ORYSA (P83649) Salt-stress root protein RS1,
partial (14%)
Length = 551
Score = 32.0 bits (71), Expect = 0.28
Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 2/75 (2%)
Frame = +3
Query: 69 EEETLMGSDRDVVPDAEASAPQDTNVSQE--NPIPENPDVEDVIAEETVSTETPSSSKKG 126
EEE DR++V + E + V++E + P E+ E V E P ++K+
Sbjct: 12 EEEASTTKDREIVVEEEKKEEEKPQVAEETTEKVESEPPKEEEKPAEPVVAEAPPAAKEE 191
Query: 127 DKGSVSSKETHSDEP 141
+K E EP
Sbjct: 192 EKPVEPVAEVEKTEP 236
>BP042644
Length = 440
Score = 32.0 bits (71), Expect = 0.28
Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 3/75 (4%)
Frame = +2
Query: 87 SAPQDTNVSQE---NPIPENPDVEDVIAEETVSTETPSSSKKGDKGSVSSKETHSDEPIQ 143
SAP+ + V +E N I + D + V ++ V E+P + +GS S P
Sbjct: 215 SAPETSQVKKEGLKNVIYIDVDNDQV--DDVVILESPEFVSRKVRGSCGPSRERSFTPQS 388
Query: 144 VLVQNNSDGDDSDDD 158
V+ + D DD DDD
Sbjct: 389 VISIDTDDDDDDDDD 433
>TC17290 similar to UP|Q8H4Y5 (Q8H4Y5) OJ1136_A10.4 protein, partial (14%)
Length = 540
Score = 31.6 bits (70), Expect = 0.37
Identities = 20/51 (39%), Positives = 29/51 (56%), Gaps = 1/51 (1%)
Frame = +2
Query: 111 AEETVST-ETPSSSKKGDKGSVSSKETHSDEPIQVLVQNNSDGDDSDDDDV 160
A T ST E SS K+ +KG+ S ++ S +N+DGD+ DDDD+
Sbjct: 233 AYSTSSTGERASSDKEDEKGNTLSGKSDSKP-------SNADGDEEDDDDL 364
>TC14217
Length = 985
Score = 31.6 bits (70), Expect = 0.37
Identities = 40/174 (22%), Positives = 71/174 (39%), Gaps = 20/174 (11%)
Frame = +1
Query: 85 EASAPQDTNVSQENPIPENPDVEDVIAEETVSTETPS---SSKKGDKGSVSSKE------ 135
E AP+ VS E VE+ E +TET S + K D +V K+
Sbjct: 76 EPEAPEAVQVSSREVEVETKKVEEQNEAEAATTETESVKVENVKNDTQAVDDKKEENADT 255
Query: 136 ------THSDEPIQVLVQNNSDGDDSDDDDVPLFETLPESVGARLKRK-RRTTASEAPSV 188
T EP++ + + ++S + V T E V +K + + T S+
Sbjct: 256 KVDEISTAISEPVRETLASKFKDEESIETGVD--NTEKEQVEEPVKTEVQDTKESDVTKT 429
Query: 189 PQKKTKPSPTTPKSKK-KDLKGKGK---ATAEKSMSEKKKKAPVIVSESDSDVE 238
+ K +P P K+ ++ K K A A+K+++ K + + E+ D++
Sbjct: 430 SKDLPKETPAKPAQKQSNNIISKVKQSLAKAKKAITGKSPSSKNLSPEAKGDIQ 591
>TC8259 similar to UP|HMGL_SOYBN (P26585) HMG1/2-like protein (SB11
protein), partial (92%)
Length = 942
Score = 31.6 bits (70), Expect = 0.37
Identities = 36/166 (21%), Positives = 68/166 (40%), Gaps = 9/166 (5%)
Frame = +2
Query: 3 LKSGSSKSTSSKTEKSLSQSRKK--ARTPAR-------KYYKSKSHGVSSSHVLLEDVTS 53
+K G S++ S + E L+ ++K A PAR K S+ V +ED
Sbjct: 176 MKGGKSRTESKRAEPKLAVNKKAGGATKPARGAKGKAAKDPNKPKRPPSAFFVFMEDFRK 355
Query: 54 DEEEEPFHDAPANINEEETLMGSDRDVVPDAEASAPQDTNVSQENPIPENPDVEDVIAEE 113
+E N E + + V A + + + +++ P + E+
Sbjct: 356 IFNKE---------NPENKAVSA----VGKAAGAKWKSMSEAEKAPYVAKAEKRKADYEK 496
Query: 114 TVSTETPSSSKKGDKGSVSSKETHSDEPIQVLVQNNSDGDDSDDDD 159
T+ + +KK +G ++ E S++ + + + D DDSD+DD
Sbjct: 497 TMK----AYNKKQAEGPAAADEEESEKSVSEVNDQDEDDDDSDEDD 622
>TC8582 similar to UP|Q8LJS2 (Q8LJS2) Nucleolar histone deacetylase
HD2-P39, partial (65%)
Length = 1341
Score = 30.0 bits (66), Expect = 1.1
Identities = 49/225 (21%), Positives = 79/225 (34%), Gaps = 8/225 (3%)
Frame = +1
Query: 7 SSKSTSSKTEKSLSQS---RKKARTPARKYYKSK----SHGVSSSHVLLEDVTSDEEEEP 59
S S S + E+ L + + + +T K K+ + G S+ V + D DEEE
Sbjct: 376 SDFSESDEEEEELMEQENGKPETKTEDAKVAKTAKPAAAAGPSAKQVKIVDPKKDEEEAD 555
Query: 60 FHDAPANINE-EETLMGSDRDVVPDAEASAPQDTNVSQENPIPENPDVEDVIAEETVSTE 118
+ +++ E+ L+ +D D S + E+ D E E
Sbjct: 556 LDVSSPDVSGYEDDLISADED---------------SDDESDDESDDEE----------E 660
Query: 119 TPSSSKKGDKGSVSSKETHSDEPIQVLVQNNSDGDDSDDDDVPLFETLPESVGARLKRKR 178
TP+ +KK D+G E+ S P+ N+ + D +K
Sbjct: 661 TPTPAKKVDQGKKRPNESASKTPVSGKKAKNATPEKPDG------------------KKN 786
Query: 179 RTTASEAPSVPQKKTKPSPTTPKSKKKDLKGKGKATAEKSMSEKK 223
TA+ P KT S KS K K+ K + K
Sbjct: 787 VHTATPHPKKQDGKTPNSFGKNKSPNSGGKFSNKSGGSKPFNSNK 921
>AV408367
Length = 431
Score = 30.0 bits (66), Expect = 1.1
Identities = 31/125 (24%), Positives = 50/125 (39%)
Frame = +3
Query: 113 ETVSTETPSSSKKGDKGSVSSKETHSDEPIQVLVQNNSDGDDSDDDDVPLFETLPESVGA 172
E + + PSS + D S+ K+ D+ +V+ + G D D ++V
Sbjct: 63 ENIRLKEPSSQLRNDSSSI--KQPKKDKGDEVIPVHAPCGHDKLDPK--------QTVSK 212
Query: 173 RLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKDLKGKGKATAEKSMSEKKKKAPVIVSE 232
K + S+P K P+ KS K LKG AT +K + K+ +S
Sbjct: 213 MAKLSSASPVKSPQSLPAMK--PNLDRNKSSKPLLKGSSNAT-QKKVDHASSKSSHNLSS 383
Query: 233 SDSDV 237
S + V
Sbjct: 384 SQNQV 398
>TC19394 similar to UP|NSR1_YEAST (P27476) Nuclear localization sequence
binding protein (P67), partial (6%)
Length = 590
Score = 29.6 bits (65), Expect = 1.4
Identities = 30/152 (19%), Positives = 65/152 (42%)
Frame = +1
Query: 90 QDTNVSQENPIPENPDVEDVIAEETVSTETPSSSKKGDKGSVSSKETHSDEPIQVLVQNN 149
+ TN +NP P ++E++S + SS ++ ++ +E +E ++ + +
Sbjct: 22 EPTNP*FQNPXAR-PSKTQSSSDESLSNSSSSSEEEQQIEQINDEE--DEEELEAVARPA 192
Query: 150 SDGDDSDDDDVPLFETLPESVGARLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKDLKG 209
S GDD +D+P P+S A+E + P+ +K++
Sbjct: 193 SSGDDEVAEDIP-----PDS-------DEDPAAAEDADDDDDEQGGDHVDPEIRKRE--- 327
Query: 210 KGKATAEKSMSEKKKKAPVIVSESDSDVEADV 241
K+ + KK+K I+ ++ ++AD+
Sbjct: 328 --KSRLREMQIMKKQKVQEILDAQNAAIDADM 417
>BP057817
Length = 545
Score = 29.3 bits (64), Expect = 1.8
Identities = 17/58 (29%), Positives = 24/58 (41%)
Frame = -2
Query: 149 NSDGDDSDDDDVPLFETLPESVGARLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKD 206
+ D D DDDD P E G R+R+T S P + PT P + ++
Sbjct: 544 DDDWSDEDDDDTP--PDFDEDAGTLEIRQRKTINQVEDSEPNNEKVLVPTLPDGRPRE 377
>TC14766 similar to UP|Q7XXR8 (Q7XXR8) Nascent polypeptide associated
complex alpha chain, partial (58%)
Length = 600
Score = 29.3 bits (64), Expect = 1.8
Identities = 30/132 (22%), Positives = 52/132 (38%), Gaps = 1/132 (0%)
Frame = +2
Query: 104 PDVEDVIAEETVSTE-TPSSSKKGDKGSVSSKETHSDEPIQVLVQNNSDGDDSDDDDVPL 162
P++ V ST T S ++ + ++ H DEP+ + D +D DD+D
Sbjct: 14 PEISSVCTGTHRSTAMTAQSQEELLAAHLEQQKIHDDEPVVEDDDEDDDDEDDDDEDDDN 193
Query: 163 FETLPESVGARLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKDLKGKGKATAEKSMSEK 222
E R K+ R +KK++ + K K + G + T +KS
Sbjct: 194 IEGQEGDASGRSKQTR----------SEKKSRKAML--KLGMKPVTGVSRVTVKKS---- 325
Query: 223 KKKAPVIVSESD 234
K ++S+ D
Sbjct: 326 -KNIVFVISKPD 358
>BP030312
Length = 418
Score = 29.3 bits (64), Expect = 1.8
Identities = 16/50 (32%), Positives = 21/50 (42%)
Frame = -1
Query: 192 KTKPSPTTPKSKKKDLKGKGKATAEKSMSEKKKKAPVIVSESDSDVEADV 241
K PSPT PKS K + G+ + K P+I EA+V
Sbjct: 334 KVTPSPTPPKSVKLNATGRSNGGVHPETTPPNTKNPLISFPCHRKTEANV 185
>AV410494
Length = 398
Score = 29.3 bits (64), Expect = 1.8
Identities = 18/48 (37%), Positives = 26/48 (53%), Gaps = 2/48 (4%)
Frame = +2
Query: 161 PLFE--TLPESVGARLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKD 206
P+ E TLP + LKRKR A +VP+ + P P+S+K+D
Sbjct: 5 PIMEAATLPPAAAPVLKRKR---GRPARAVPKTTSAAPPPPPRSQKRD 139
>TC10270 homologue to UP|Q8VXT0 (Q8VXT0) Translation initiation factor
(eIF-1A), partial (98%)
Length = 619
Score = 28.9 bits (63), Expect = 2.4
Identities = 17/59 (28%), Positives = 27/59 (44%)
Frame = +1
Query: 183 SEAPSVPQKKTKPSPTTPKSKKKDLKGKGKATAEKSMSEKKKKAPVIVSESDSDVEADV 241
S S P+K P P +P + K+ KGKG ++ +E + +V + D A V
Sbjct: 43 SREQSPPEKAVSPPPPSPTTMPKN-KGKGGKNRKRGKNEADDEKRELVFKEDGQEYAQV 216
>TC8736 similar to PIR|T06396|T06396 isoprenylated protein - soybean
(fragment) {Glycine max;}, partial (84%)
Length = 1268
Score = 28.9 bits (63), Expect = 2.4
Identities = 25/86 (29%), Positives = 34/86 (39%), Gaps = 5/86 (5%)
Frame = +2
Query: 186 PSVPQKKTKPSPTTPKS----KKKD-LKGKGKATAEKSMSEKKKKAPVIVSESDSDVEAD 240
P P+ + K PK+ KKKD K K A EK +K KA + D D E
Sbjct: 467 PKPPEPEKKKEADKPKAAEHEKKKDGEKPKAAAEPEKKKDSEKPKAAEPEKKKDGDKEKP 646
Query: 241 VPDIVLAEMKKFAGRRIPKNVPAAPL 266
D +K + P +P P+
Sbjct: 647 KSDGPKKGDEKPKDKPAPGPIPVHPV 724
>BP042908
Length = 327
Score = 28.9 bits (63), Expect = 2.4
Identities = 17/50 (34%), Positives = 22/50 (44%), Gaps = 2/50 (4%)
Frame = -3
Query: 161 PLFETLPESV--GARLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKDLK 208
P F L ES+ R K T + S P PSP++PKSK +
Sbjct: 220 PCFPNLSESLLQAVRQSGKSGTQPAATRSSPSASKAPSPSSPKSKSSSAR 71
>AV780534
Length = 536
Score = 28.5 bits (62), Expect = 3.1
Identities = 28/137 (20%), Positives = 53/137 (38%)
Frame = +1
Query: 110 IAEETVSTETPSSSKKGDKGSVSSKETHSDEPIQVLVQNNSDGDDSDDDDVPLFETLPES 169
+ TV TETPSS ++KET + I N + + + P+
Sbjct: 160 VLSNTVITETPSS---------ANKETSECKSI-----NFQPLEPKETSECTSINVQPQE 297
Query: 170 VGARLKRKRRTTASEAPSVPQKKTKPSPTTPKSKKKDLKGKGKATAEKSMSEKKKKAPVI 229
K + + P + + + TT S +K + + E+ + + I
Sbjct: 298 PNVAEKNNTTNFSDDKPQLTSNVAEKNNTTDFSDEK---------PQLTSMERSELSETI 450
Query: 230 VSESDSDVEADVPDIVL 246
V+E++ V+ VP+ V+
Sbjct: 451 VTETECKVDVSVPESVV 501
>BP038767
Length = 561
Score = 28.5 bits (62), Expect = 3.1
Identities = 16/48 (33%), Positives = 26/48 (53%), Gaps = 3/48 (6%)
Frame = +2
Query: 161 PLFETLPESVGARLKRKRRTTASEAPS---VPQKKTKPSPTTPKSKKK 205
PL T+P++ + + T ++ PS +P+K PSP+T S KK
Sbjct: 236 PLRITMPQTQTPTRRSEPAATNNKTPSPSPLPKKPPSPSPSTRSSAKK 379
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.127 0.347
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,911,735
Number of Sequences: 28460
Number of extensions: 96479
Number of successful extensions: 629
Number of sequences better than 10.0: 65
Number of HSP's better than 10.0 without gapping: 609
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 625
length of query: 577
length of database: 4,897,600
effective HSP length: 95
effective length of query: 482
effective length of database: 2,193,900
effective search space: 1057459800
effective search space used: 1057459800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0280b.3