BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0216.15
(500 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW428847 79 1e-15
TC13431 47 6e-06
BP036259 45 2e-05
BP056980 38 0.003
AV766106 35 0.037
TC19700 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like prot... 32 0.31
TC11793 similar to UP|AAR99376 (AAR99376) Ring domain containing... 30 0.91
TC9502 similar to UP|Q8LG88 (Q8LG88) Sodium-dicarboxylate cotran... 30 0.91
TC12668 similar to UP|AAQ84334 (AAQ84334) Zinc-finger protein, p... 30 1.2
TC9417 weakly similar to UP|Q9S834 (Q9S834) ATP-dependent Clp pr... 29 1.6
TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, parti... 29 2.0
TC14157 similar to UP|O24080 (O24080) Peroxidase2 precursor , p... 29 2.0
TC13668 29 2.0
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 28 2.7
CB828968 28 3.5
BP063315 28 4.5
AV775706 28 4.5
TC7976 homologue to UP|METK_LYCES (P43280) S-adenosylmethionine ... 28 4.5
TC13730 weakly similar to UP|Q9LTT8 (Q9LTT8) Gb|AAF53066.1, part... 27 5.9
TC19557 similar to PIR|E86326|E86326 protein F18O14.3 [imported]... 27 5.9
>AW428847
Length = 473
Score = 79.3 bits (194), Expect = 1e-15
Identities = 46/126 (36%), Positives = 66/126 (51%), Gaps = 8/126 (6%)
Frame = +2
Query: 261 DKSTSENGKWILAHTKPCPRCKSLIEKNQGFKYMTC--KCGFQFCWVCF-----HEQLKC 313
+ + SEN WILA++KPCP+CK IEKNQG +MTC C F+FCW+C H +
Sbjct: 23 NSAESENMNWILANSKPCPKCKRPIEKNQGCMHMTCTPPCKFEFCWLCLGAWSDHGERTG 202
Query: 314 ESRSCSPFIRMHIMEFQGT-DGEEVEINMEKDNPKKCNHYYECWARNDFLSKSILQKLID 372
+C+ R + +G D E M K++ ++ HYYE WA N + L L
Sbjct: 203 GFYACN---RYETAKQEGVYDETERRREMAKNSLERYTHYYERWASNQSSRQKALADLHQ 373
Query: 373 LDRKYL 378
+ +L
Sbjct: 374 MQTIHL 391
>TC13431
Length = 552
Score = 47.4 bits (111), Expect = 6e-06
Identities = 22/78 (28%), Positives = 38/78 (48%), Gaps = 3/78 (3%)
Frame = +3
Query: 232 CYHSFCWRCGEESHSPVDCETAGRWMKKIDKSTSENGKWILAHTKP---CPRCKSLIEKN 288
C+ FC +C H+ ++CE + + + E LA K CP+C +++
Sbjct: 24 CHRLFCAQCMVPWHAGMNCEEFQKSGRVKGEEDLEKKFLKLAKRKKWQRCPKCSFYVQRR 203
Query: 289 QGFKYMTCKCGFQFCWVC 306
G ++M C+CG FC+ C
Sbjct: 204 SGCEHMKCRCGCNFCYEC 257
Score = 33.1 bits (74), Expect = 0.11
Identities = 24/88 (27%), Positives = 37/88 (41%)
Frame = +3
Query: 156 CLTLRCPDPSCDVAVDRDMIRELASESSKVMYEQFLLWSYVDSKAKMRWCPAPGCDYVVC 215
C L C C V M E +S +V E+ L ++ + +W P C + V
Sbjct: 24 CHRLFCAQ--CMVPWHAGMNCEEFQKSGRVKGEEDLEKKFLKLAKRKKWQRCPKCSFYV- 194
Query: 216 YQGDGGCARDLDVTCLCYHSFCWRCGEE 243
Q GC + C C +FC+ CG++
Sbjct: 195 -QRRSGCEH---MKCRCGCNFCYECGKD 266
>BP036259
Length = 532
Score = 45.4 bits (106), Expect = 2e-05
Identities = 24/85 (28%), Positives = 39/85 (45%), Gaps = 10/85 (11%)
Frame = -2
Query: 232 CYHSFCWRCGEESHSPVDCETAGRWMKKID----------KSTSENGKWILAHTKPCPRC 281
C FC+ C H+ + C KK++ KS + W + C +C
Sbjct: 459 CRGFFCFICKVPWHNGMTCHA----YKKLNPNPPAEDLKLKSLATRSLW-----RQCVKC 307
Query: 282 KSLIEKNQGFKYMTCKCGFQFCWVC 306
+IE +G +MTC+CG++FC+ C
Sbjct: 306 NHMIELAEGCYHMTCRCGYEFCYQC 232
>BP056980
Length = 451
Score = 38.1 bits (87), Expect = 0.003
Identities = 14/31 (45%), Positives = 18/31 (57%)
Frame = -1
Query: 276 KPCPRCKSLIEKNQGFKYMTCKCGFQFCWVC 306
K C C +IEK G ++ C+CG CWVC
Sbjct: 397 KSCSACGYVIEKVDGCHHVECQCGKHVCWVC 305
>AV766106
Length = 432
Score = 34.7 bits (78), Expect = 0.037
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -3
Query: 115 CQVCFRSSEKGKILSAGCAHPLCEACW 141
C +CF ++ + +I A C HP C +CW
Sbjct: 337 CGICFENNPRARIEVAFCGHPHCFSCW 257
>TC19700 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like protein,
partial (25%)
Length = 442
Score = 31.6 bits (70), Expect = 0.31
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +3
Query: 270 WILAHTKPCPRCKSLIEKNQGFKYMTCKCGFQFCW 304
W+ AHT +L+E + G++ +C C F FCW
Sbjct: 330 WVGAHT-------ALLESSAGWRSASCWCQFCFCW 413
>TC11793 similar to UP|AAR99376 (AAR99376) Ring domain containing protein,
partial (23%)
Length = 527
Score = 30.0 bits (66), Expect = 0.91
Identities = 24/81 (29%), Positives = 36/81 (43%), Gaps = 1/81 (1%)
Frame = +3
Query: 209 GCDYVVCYQGDGGCARDLDVTCLCYHSFCWRCGEESHSPVDCETAGRWMKKIDKSTSENG 268
G D +C C +D VT LC H +CW C +W+ + ++SEN
Sbjct: 342 GFDCNICLD----CVQDPVVT-LCGHLYCWPC------------IYKWLNS-NSASSEN- 464
Query: 269 KWILAHTKP-CPRCKSLIEKN 288
+ KP CP CKS + ++
Sbjct: 465 ---VEQNKPQCPVCKSEVSQS 518
>TC9502 similar to UP|Q8LG88 (Q8LG88) Sodium-dicarboxylate
cotransporter-like, partial (42%)
Length = 1253
Score = 30.0 bits (66), Expect = 0.91
Identities = 16/51 (31%), Positives = 20/51 (38%)
Frame = +1
Query: 160 RCPDPSCDVAVDRDMIRELASESSKVMYEQFLLWSYVDSKAKMRWCPAPGC 210
R S D +RD IR LAS + W Y + WC + GC
Sbjct: 481 RASSSSYDSRSNRDRIRFLASNFNSFQCSWICHWPY*NPGHAQSWCASKGC 633
>TC12668 similar to UP|AAQ84334 (AAQ84334) Zinc-finger protein, partial
(37%)
Length = 513
Score = 29.6 bits (65), Expect = 1.2
Identities = 19/43 (44%), Positives = 23/43 (53%)
Frame = +2
Query: 261 DKSTSENGKWILAHTKPCPRCKSLIEKNQGFKYMTCKCGFQFC 303
D S+ EN + + A T P RC S K GF +CKCG FC
Sbjct: 113 DSSSGENSE-VKAKTGP-NRCVSC-RKRVGFTGFSCKCGNLFC 232
>TC9417 weakly similar to UP|Q9S834 (Q9S834) ATP-dependent Clp protease
subunit ClpP (ATP-dependent Clp protease proteolytic
subunit ClpP5) (Endopeptidase Clp) , partial (14%)
Length = 466
Score = 29.3 bits (64), Expect = 1.6
Identities = 21/77 (27%), Positives = 36/77 (46%)
Frame = +1
Query: 20 ERDKNLPNTEEEEEDKRENFTILSELEIKRLQEDAINKVASVLCIPDASACFLLCHNLWN 79
E + L EEEEE+ RE + E+ +K +N+ +CI S C
Sbjct: 229 EGKERLTEREEEEEEGREGRNVREEVGVKSDVCAIVNEGEEAICI-YLSMC--------- 378
Query: 80 VTKVLESWCEDEEKVQK 96
++K+ W E ++K++K
Sbjct: 379 ISKLRLLWIERQKKMKK 429
>TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, partial (81%)
Length = 1294
Score = 28.9 bits (63), Expect = 2.0
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = +3
Query: 215 CYQGDGGCARDLDVTCLCYHSFC 237
C+QG GGC+ ++ C C C
Sbjct: 666 CWQGQGGCSGGSEICCQC*EEHC 734
>TC14157 similar to UP|O24080 (O24080) Peroxidase2 precursor , partial
(96%)
Length = 1390
Score = 28.9 bits (63), Expect = 2.0
Identities = 23/84 (27%), Positives = 30/84 (35%), Gaps = 5/84 (5%)
Frame = +3
Query: 230 CLCYHSFCWRCGEESHSPVDCETAGRWMKKIDKSTSENGKWILAHTKPCPRCKSLIEKNQ 289
C CY W SP T G W++ D+S S G RC ++ E Q
Sbjct: 273 CFCYGGV*WE*QSREGSP**SVTGG*WVRHRDQSESRRG-----------RCSAVQE--Q 413
Query: 290 GFKYMTCKCGFQFC-----WVCFH 308
GF + G+ C W H
Sbjct: 414 GFVC*YSRLGYPGCCCSGWWTFLH 485
>TC13668
Length = 662
Score = 28.9 bits (63), Expect = 2.0
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 289 QGFKYMTCKCGFQFCWVC 306
+G YM C+CG FC+ C
Sbjct: 1 EGCMYMKCRCGHAFCYNC 54
Score = 27.7 bits (60), Expect = 4.5
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +1
Query: 230 CLCYHSFCWRCGEESHSP 247
C C H+FC+ CG + +P
Sbjct: 22 CRCGHAFCYNCGAPNKNP 75
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 28.5 bits (62), Expect = 2.7
Identities = 17/62 (27%), Positives = 28/62 (44%)
Frame = +1
Query: 206 PAPGCDYVVCYQGDGGCARDLDVTCLCYHSFCWRCGEESHSPVDCETAGRWMKKIDKSTS 265
P PG DG ARD + + C+RCGE H +C+ + + + + +S S
Sbjct: 370 PPPGSGRCFNCGIDGHWARDCKAGD--WKNKCYRCGERGHIEKNCKNSPKKLSRHPRSDS 543
Query: 266 EN 267
+
Sbjct: 544 RS 549
>CB828968
Length = 572
Score = 28.1 bits (61), Expect = 3.5
Identities = 10/31 (32%), Positives = 21/31 (67%)
Frame = -2
Query: 370 LIDLDRKYLSMSLQKRETYFDFVKKAWQQIA 400
L+DLD ++ ++L++ +++ AWQQ+A
Sbjct: 244 LVDLDDQHKDLNLRRHTHRLNYLTAAWQQLA 152
>BP063315
Length = 440
Score = 27.7 bits (60), Expect = 4.5
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +3
Query: 19 RERDKNLPNTEEEEEDKRENFTILSELEIKRLQE 52
RER++ EEEE++RE + +E+E R+ E
Sbjct: 9 REREREREREREEEEEEREEKNLETEVEELRVLE 110
>AV775706
Length = 462
Score = 27.7 bits (60), Expect = 4.5
Identities = 15/68 (22%), Positives = 31/68 (45%), Gaps = 10/68 (14%)
Frame = -2
Query: 91 EEKVQKAAGLLNQPKVEIDWL---------KDLCQVCFRSSE-KGKILSAGCAHPLCEAC 140
E +QK L + +E WL + +C VC + K ++++ C+H AC
Sbjct: 392 EVSLQKKDAGLGRKLLESSWLLRGTSPKKERKVCAVCLEDFQPKQQVMNLSCSHTYHSAC 213
Query: 141 WMDYIDTN 148
+ +++ +
Sbjct: 212 LLPWLEAH 189
>TC7976 homologue to UP|METK_LYCES (P43280) S-adenosylmethionine synthetase
1 (Methionine adenosyltransferase 1) (AdoMet synthetase
1) , partial (67%)
Length = 862
Score = 27.7 bits (60), Expect = 4.5
Identities = 15/45 (33%), Positives = 21/45 (46%)
Frame = +1
Query: 193 WSYVDSKAKMRWCPAPGCDYVVCYQGDGGCARDLDVTCLCYHSFC 237
W + D +A WC PG +CY+ D D C CY ++C
Sbjct: 388 WPF-DQEA*GNWCW*PGSHVWLCYR*DP*A--DASEPCPCYQAWC 513
>TC13730 weakly similar to UP|Q9LTT8 (Q9LTT8) Gb|AAF53066.1, partial (10%)
Length = 493
Score = 27.3 bits (59), Expect = 5.9
Identities = 16/47 (34%), Positives = 24/47 (51%), Gaps = 1/47 (2%)
Frame = -2
Query: 273 AHTKPC-PRCKSLIEKNQGFKYMTCKCGFQFCWVCFHEQLKCESRSC 318
+H + C PR K+L+ ++ FK+ KC F LKC +R C
Sbjct: 486 SHCQ*CRPRFKALLHRS*MFKHAFLKCRIHLFKHGFTCHLKCRNR*C 346
>TC19557 similar to PIR|E86326|E86326 protein F18O14.3 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(24%)
Length = 457
Score = 27.3 bits (59), Expect = 5.9
Identities = 17/62 (27%), Positives = 24/62 (38%)
Frame = +2
Query: 228 VTCLCYHSFCWRCGEESHSPVDCETAGRWMKKIDKSTSENGKWILAHTKPCPRCKSLIEK 287
V LC H FCW C RW+ +S + CP CK+LI++
Sbjct: 344 VITLCGHLFCWPC------------LYRWLHHHSQS------------QECPVCKALIQE 451
Query: 288 NQ 289
+
Sbjct: 452 EK 457
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.439
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,263,163
Number of Sequences: 28460
Number of extensions: 200342
Number of successful extensions: 1224
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1181
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1215
length of query: 500
length of database: 4,897,600
effective HSP length: 94
effective length of query: 406
effective length of database: 2,222,360
effective search space: 902278160
effective search space used: 902278160
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 57 (26.6 bits)
Lotus: description of TM0216.15