BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0133.10
(382 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV426390 31 0.003
AV408212 37 0.005
BP043633 35 0.027
AV415922 34 0.035
TC16241 weakly similar to UP|Q91TH4 (Q91TH4) T122, partial (7%) 33 0.061
TC15704 similar to UP|O22265 (O22265) Expressed protein (At2g474... 33 0.061
TC15341 similar to UP|Q9M0H8 (Q9M0H8) Predicted proline-rich pro... 33 0.10
AV779571 32 0.13
TC12971 similar to GB|AAN73294.1|25141199|BT002297 At5g12010/F14... 32 0.23
TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycop... 31 0.39
TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fra... 31 0.39
BP057889 31 0.39
TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transc... 30 0.51
AV410007 30 0.51
TC8185 similar to UP|Q9FLV3 (Q9FLV3) Similarity to ribosomal pro... 30 0.51
TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protei... 30 0.67
TC11114 similar to UP|ELS_MOUSE (P54320) Elastin precursor (Trop... 30 0.67
AV418552 30 0.67
BP043764 30 0.67
TC19911 weakly similar to PIR|A44805|A44805 eggshell protein pre... 30 0.87
>AV426390
Length = 399
Score = 30.8 bits (68), Expect(2) = 0.003
Identities = 17/36 (47%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
Frame = +3
Query: 123 MKEASEQQQQQQLQQQQQQRAPDSPQL---SQNPSF 155
M +++QQQ QQ QQQQ PD L S +PSF
Sbjct: 54 MSLSNQQQQPQQQQQQQHHSHPDCASLIANSHSPSF 161
Score = 25.8 bits (55), Expect(2) = 0.003
Identities = 11/35 (31%), Positives = 18/35 (51%)
Frame = +1
Query: 185 SSYPPAFVPNSYPVPVASPIAPPPLMAENPNFILP 219
SS P +P+ P V + PP++ P ++LP
Sbjct: 133 SSLTPTLLPS--PTSVTGNLITPPILFSPPRYVLP 231
>AV408212
Length = 362
Score = 37.0 bits (84), Expect = 0.005
Identities = 29/85 (34%), Positives = 40/85 (46%)
Frame = +3
Query: 73 QSQQSTQQPQQHKKQVRRRLHTSRPYQERLLNMAEARREIVTALKFHRASMKEASEQQQQ 132
Q QQS Q QQ ++ ++ Q+ LL A++++ +QQQQ
Sbjct: 3 QPQQSPHQQQQQQQHMQM--------QQILLQ--------------RHAALQQQQQQQQQ 116
Query: 133 QQLQQQQQQRAPDSPQLSQNPSFDQ 157
QQ QQQQQQ PQ SQ S D+
Sbjct: 117 QQQQQQQQQSQQQQPQ-SQQQSRDR 188
Score = 31.2 bits (69), Expect = 0.30
Identities = 15/31 (48%), Positives = 16/31 (51%)
Frame = +3
Query: 130 QQQQQLQQQQQQRAPDSPQLSQNPSFDQDGR 160
QQQQQ QQQQQQ+ Q P Q R
Sbjct: 90 QQQQQQQQQQQQQQQQQQSQQQQPQSQQQSR 182
Score = 31.2 bits (69), Expect = 0.30
Identities = 15/31 (48%), Positives = 19/31 (60%)
Frame = +3
Query: 128 EQQQQQQLQQQQQQRAPDSPQLSQNPSFDQD 158
+QQQQQQ QQQQQQ+ Q Q+ +D
Sbjct: 93 QQQQQQQQQQQQQQQQQSQQQQPQSQQQSRD 185
>BP043633
Length = 542
Score = 34.7 bits (78), Expect = 0.027
Identities = 26/97 (26%), Positives = 42/97 (42%), Gaps = 1/97 (1%)
Frame = +3
Query: 168 RIYPSCNTNFSSYMGDFSSYPPAFVPNSYPVPVASPIAPPPLMAENP-NFILPNQPLGLN 226
R++ S T +S S PP F PN+ +SP+ PP+ P P+ +G
Sbjct: 228 RLHASSTTTSASASAA*SQPPPPFPPNTTATSPSSPVVLPPISVPPPIPDSFPSTAVGAT 407
Query: 227 LNFHDFNNLDATIHLSNTSLSSNSFSSATSSSQEVPS 263
+ + S SS+S SS++ +S VP+
Sbjct: 408 TTAASSSVEPLFLPPQAVSSSSSSSSSSSPASSSVPT 518
>AV415922
Length = 422
Score = 34.3 bits (77), Expect = 0.035
Identities = 20/44 (45%), Positives = 26/44 (58%), Gaps = 4/44 (9%)
Frame = +3
Query: 116 LKFHRASMKEASEQQQQQQLQQQQQQRAPD----SPQLSQNPSF 155
++F ++ +QQQQQQ QQQQQQ+ P SP L PSF
Sbjct: 150 IQFQGNQHQQR*QQQQQQQQQQQQQQQQPSNSQ*SPLLLPFPSF 281
>TC16241 weakly similar to UP|Q91TH4 (Q91TH4) T122, partial (7%)
Length = 799
Score = 33.5 bits (75), Expect = 0.061
Identities = 31/95 (32%), Positives = 39/95 (40%), Gaps = 2/95 (2%)
Frame = +1
Query: 127 SEQQQQQQLQQQQQQRAPDS-PQLSQNPSFDQDGRYKSRRNPRIYPSCNTNFSSYMGDFS 185
S Q L + +P S P ++Q P S P +TN S S
Sbjct: 142 SPSSQPPYLSTPPKSNSPTSQPPVAQTPRAVTPISSPSSSPTSNSPYPSTNPPSLAPSIS 321
Query: 186 SYPPAFVPNSYPVP-VASPIAPPPLMAENPNFILP 219
+ PPA P S PVP ASP + P AE+P LP
Sbjct: 322 TSPPALTPASTPVPATASPPSQSP-SAESPGASLP 423
>TC15704 similar to UP|O22265 (O22265) Expressed protein
(At2g47450/T30B22.25), partial (25%)
Length = 664
Score = 33.5 bits (75), Expect = 0.061
Identities = 28/83 (33%), Positives = 33/83 (39%), Gaps = 2/83 (2%)
Frame = -1
Query: 143 APDSP--QLSQNPSFDQDGRYKSRRNPRIYPSCNTNFSSYMGDFSSYPPAFVPNSYPVPV 200
AP SP QLS P RNP P T+ S PNS+P P
Sbjct: 304 APTSPHPQLSGQPPPPPPRTPAPPRNPSPDPPPQTSHSPTRFHHRPSTARDTPNSHPSP- 128
Query: 201 ASPIAPPPLMAENPNFILPNQPL 223
++PI+PPP P P PL
Sbjct: 127 SAPISPPPPRTRTPPLPKP*PPL 59
>TC15341 similar to UP|Q9M0H8 (Q9M0H8) Predicted proline-rich protein,
partial (33%)
Length = 1002
Score = 32.7 bits (73), Expect = 0.10
Identities = 19/48 (39%), Positives = 28/48 (57%)
Frame = +1
Query: 124 KEASEQQQQQQLQQQQQQRAPDSPQLSQNPSFDQDGRYKSRRNPRIYP 171
++ +QQQQQQ QQQ Q+ P Q +Q PS Q + + +P +YP
Sbjct: 856 QQPQQQQQQQQQQQQWPQQLPQQVQPTQPPSM-QSPQIRPPSSP-VYP 993
Score = 28.9 bits (63), Expect = 1.5
Identities = 19/58 (32%), Positives = 30/58 (50%)
Frame = +1
Query: 123 MKEASEQQQQQQLQQQQQQRAPDSPQLSQNPSFDQDGRYKSRRNPRIYPSCNTNFSSY 180
+++ + QQ QQ QQQQQQ+ QL Q Q + S ++P+I P + + Y
Sbjct: 835 VQQYPQYQQPQQQQQQQQQQQQWPQQLPQQV---QPTQPPSMQSPQIRPPSSPVYPPY 999
Score = 28.9 bits (63), Expect = 1.5
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +1
Query: 117 KFHRASMKEASEQQQQQQLQQQQQQRAPDSPQLSQNP 153
++ + ++ +QQQQQ QQ QQ P P Q+P
Sbjct: 850 QYQQPQQQQQQQQQQQQWPQQLPQQVQPTQPPSMQSP 960
>AV779571
Length = 538
Score = 32.3 bits (72), Expect = 0.13
Identities = 17/65 (26%), Positives = 33/65 (50%)
Frame = +1
Query: 85 KKQVRRRLHTSRPYQERLLNMAEARREIVTALKFHRASMKEASEQQQQQQLQQQQQQRAP 144
+ Q ++ ++ Q+ L + + ++ + F+ + QQQQQQ QQQ QQ+
Sbjct: 133 QSQPQQVFQNNQENQQLLQSQSHLQQHLQHQHSFNGQTQNHNHNQQQQQQQQQQTQQQVV 312
Query: 145 DSPQL 149
D+ Q+
Sbjct: 313 DNQQI 327
>TC12971 similar to GB|AAN73294.1|25141199|BT002297 At5g12010/F14F18_180
{Arabidopsis thaliana;}, partial (7%)
Length = 721
Score = 31.6 bits (70), Expect = 0.23
Identities = 19/37 (51%), Positives = 23/37 (61%), Gaps = 4/37 (10%)
Frame = +2
Query: 109 RREIVTALKFHRASM----KEASEQQQQQQLQQQQQQ 141
RR +T L+ H A+ E +QQQQQQLQQQQ Q
Sbjct: 92 RRPRITFLRLHLATKFI*PNE*PQQQQQQQLQQQQ*Q 202
>TC18379 similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich glycoprotein
DZ-HRGP precursor, partial (8%)
Length = 584
Score = 30.8 bits (68), Expect = 0.39
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +1
Query: 187 YPPAFVPNSYPVPVASPIAPPPLMAENP 214
YPP P P P +P APPP A P
Sbjct: 235 YPPPMAPPPPPPPAPAPPAPPPYWAAPP 318
>TC15540 similar to UP|Q9NGS5 (Q9NGS5) Prespore protein MF12 (Fragment),
partial (6%)
Length = 806
Score = 30.8 bits (68), Expect = 0.39
Identities = 20/76 (26%), Positives = 34/76 (44%)
Frame = +2
Query: 73 QSQQSTQQPQQHKKQVRRRLHTSRPYQERLLNMAEARREIVTALKFHRASMKEASEQQQQ 132
Q Q Q PQ + Q +++ + Q++L M + + + + + + QQQ
Sbjct: 26 QQQLQQQPPQLTQLQQQQQQQLPQVQQQQLSQMQQQQLP-----QLQQQQLSQLQPQQQL 190
Query: 133 QQLQQQQQQRAPDSPQ 148
QLQQ Q Q+ P Q
Sbjct: 191 PQLQQLQHQQLPQQQQ 238
Score = 28.1 bits (61), Expect = 2.5
Identities = 20/70 (28%), Positives = 33/70 (46%), Gaps = 1/70 (1%)
Frame = +2
Query: 73 QSQQSTQQPQQHKKQVRRRLHTSRPY-QERLLNMAEARREIVTALKFHRASMKEASEQQQ 131
Q QQ Q PQ ++Q+ + P Q++ L+ + ++++ +Q Q
Sbjct: 68 QQQQQQQLPQVQQQQLSQMQQQQLPQLQQQQLSQLQPQQQLPQL------------QQLQ 211
Query: 132 QQQLQQQQQQ 141
QQL QQQQQ
Sbjct: 212 HQQLPQQQQQ 241
Score = 26.9 bits (58), Expect = 5.7
Identities = 15/25 (60%), Positives = 16/25 (64%), Gaps = 2/25 (8%)
Frame = +2
Query: 129 QQQQQQLQQQQQQRAP--DSPQLSQ 151
Q QQQQL Q QQQ+ P QLSQ
Sbjct: 95 QVQQQQLSQMQQQQLPQLQQQQLSQ 169
Score = 26.6 bits (57), Expect = 7.4
Identities = 15/35 (42%), Positives = 20/35 (56%), Gaps = 7/35 (20%)
Frame = +2
Query: 124 KEASEQQQQQQLQQQ-------QQQRAPDSPQLSQ 151
++ + QQQQQLQQQ QQQ+ PQ+ Q
Sbjct: 2 QQLPQLQQQQQLQQQPPQLTQLQQQQQQQLPQVQQ 106
Score = 26.6 bits (57), Expect = 7.4
Identities = 15/30 (50%), Positives = 17/30 (56%)
Frame = +2
Query: 128 EQQQQQQLQQQQQQRAPDSPQLSQNPSFDQ 157
+QQQQQQL Q QQQ+ Q Q P Q
Sbjct: 68 QQQQQQQLPQVQQQQLSQMQQ-QQLPQLQQ 154
>BP057889
Length = 473
Score = 30.8 bits (68), Expect = 0.39
Identities = 19/62 (30%), Positives = 26/62 (41%)
Frame = -3
Query: 146 SPQLSQNPSFDQDGRYKSRRNPRIYPSCNTNFSSYMGDFSSYPPAFVPNSYPVPVASPIA 205
+PQ S G Y + P P + +Y + PPA P +Y PVA+P
Sbjct: 471 NPQASYGTYPGYGGHYANPPLPTSVPPNTAAYGAYPPAYP-VPPAVPPPTYVPPVAAPTQ 295
Query: 206 PP 207
PP
Sbjct: 294 PP 289
>TC16093 weakly similar to UP|PF21_ARATH (Q04088) Possible transcription
factor PosF21 (AtbZIP59), partial (17%)
Length = 570
Score = 30.4 bits (67), Expect = 0.51
Identities = 29/125 (23%), Positives = 50/125 (39%)
Frame = +1
Query: 48 GCVVNSDASSHGQNLTKHGKIGKACQSQQSTQQPQQHKKQVRRRLHTSRPYQERLLNMAE 107
G ++N + GQ H + Q QQ Q H ++ ++ H +Q+ + +
Sbjct: 1 GPMMNYASFGGGQQFYPHNHSMHTLLAAQQFQQLQIHPQKHQQHQHQ---FQQPIQQQQQ 171
Query: 108 ARREIVTALKFHRASMKEASEQQQQQQLQQQQQQRAPDSPQLSQNPSFDQDGRYKSRRNP 167
+++ + QQ QQQ+QQQQQ++ S L S K +P
Sbjct: 172 QQQQPI---------------QQPQQQMQQQQQEQHQQSGDLKMR-SATSSPSPKDNDSP 303
Query: 168 RIYPS 172
+ PS
Sbjct: 304 DLNPS 318
>AV410007
Length = 425
Score = 30.4 bits (67), Expect = 0.51
Identities = 22/68 (32%), Positives = 35/68 (51%)
Frame = +3
Query: 88 VRRRLHTSRPYQERLLNMAEARREIVTALKFHRASMKEASEQQQQQQLQQQQQQRAPDSP 147
++RR+ TS+ R N +A +A K + K + + QQQ+ QQQQ+Q+A
Sbjct: 96 MKRRVPTSKTAMPRH-NPQKAPTNFNSATKTNNVKPKFTNTKAQQQRQQQQQRQQAKQQ- 269
Query: 148 QLSQNPSF 155
Q P+F
Sbjct: 270 QNGSRPTF 293
>TC8185 similar to UP|Q9FLV3 (Q9FLV3) Similarity to ribosomal protein 30S
subunit, partial (55%)
Length = 1268
Score = 30.4 bits (67), Expect = 0.51
Identities = 37/136 (27%), Positives = 56/136 (40%), Gaps = 17/136 (12%)
Frame = -3
Query: 196 YPVPVASPIAPPPLMAENPNFILPNQPLGLN----------------LNFHDFNNLDATI 239
Y +P PI P + +F L L LN L+ HD L
Sbjct: 666 YRLPNLRPIEPFHVATVIRHFFLDLPQLSLNDGRHQIYTCIGSFRILLSPHDAMPLGKQS 487
Query: 240 HLSNTSLSSNSFSSATSSSQEVPSVELSQGDGISSLVNSTESNAASQVNAGLHAAMDEEA 299
HL++TS S+ S+TSS + P + LS D ++ L+N + LH +D
Sbjct: 486 HLTSTSSGSSPKLSSTSSRK--PDINLS--DYVAVLLNGFP-------HLVLHVLLDSVG 340
Query: 300 IAEIRSLGEQYQ-MEW 314
EI +L +++ EW
Sbjct: 339 EVEILALDDEFDGGEW 292
>TC17113 weakly similar to UP|Q9LFU8 (Q9LFU8) Proline-rich protein, partial
(25%)
Length = 1077
Score = 30.0 bits (66), Expect = 0.67
Identities = 14/37 (37%), Positives = 21/37 (55%), Gaps = 3/37 (8%)
Frame = +1
Query: 187 YPPAFVPNSYPVP-VASPIAPPPLMAE--NPNFILPN 220
+PP +PN + P V +P PPPL+ P ++PN
Sbjct: 400 FPPPLIPNPFQPPLVPNPFQPPPLIPNPFQPPPLIPN 510
Score = 28.9 bits (63), Expect = 1.5
Identities = 11/37 (29%), Positives = 18/37 (47%)
Frame = +1
Query: 184 FSSYPPAFVPNSYPVPVASPIAPPPLMAENPNFILPN 220
F PP +P+ + P P+ P P +P ++PN
Sbjct: 514 FQPSPPPLLPDPFQPPSPPPLFPNPFQPPSPPPLIPN 624
>TC11114 similar to UP|ELS_MOUSE (P54320) Elastin precursor (Tropoelastin),
partial (3%)
Length = 517
Score = 30.0 bits (66), Expect = 0.67
Identities = 13/28 (46%), Positives = 14/28 (49%)
Frame = +1
Query: 188 PPAFVPNSYPVPVASPIAPPPLMAENPN 215
PP P +YP PI PPPL PN
Sbjct: 322 PPTPTPATYPPIPNPPITPPPLTMTTPN 405
>AV418552
Length = 386
Score = 30.0 bits (66), Expect = 0.67
Identities = 24/78 (30%), Positives = 39/78 (49%), Gaps = 2/78 (2%)
Frame = +2
Query: 76 QSTQQPQQHKKQVRRRLHTSRPYQERL--LNMAEARREIVTALKFHRASMKEASEQQQQQ 133
+ + Q QQ +Q ++ L +P Q+ L L + + ++ A + S + + QQQQ
Sbjct: 41 EPSSQEQQLMQQHQQMLSYFQPQQQLLPHLQLQQQLQQQQLAQLQQQLSQLQLTLLQQQQ 220
Query: 134 QLQQQQQQRAPDSPQLSQ 151
Q QQQQ A QL+Q
Sbjct: 221 LTQLQQQQLAQLQQQLAQ 274
Score = 28.9 bits (63), Expect = 1.5
Identities = 21/84 (25%), Positives = 38/84 (45%), Gaps = 3/84 (3%)
Frame = +2
Query: 60 QNLTKHGKIGKACQSQQSTQQPQQHKK---QVRRRLHTSRPYQERLLNMAEARREIVTAL 116
Q L H ++ + Q QQ Q QQ + + ++ ++ Q++L + + ++
Sbjct: 110 QQLLPHLQLQQQLQQQQLAQLQQQLSQLQLTLLQQQQLTQLQQQQLAQLQQQLAQLQLTQ 289
Query: 117 KFHRASMKEASEQQQQQQLQQQQQ 140
H ++ QQQ QQL Q QQ
Sbjct: 290 HQHTQLQQQLLPQQQFQQLPQHQQ 361
Score = 26.2 bits (56), Expect = 9.7
Identities = 25/103 (24%), Positives = 43/103 (41%)
Frame = +2
Query: 55 ASSHGQNLTKHGKIGKACQSQQSTQQPQQHKKQVRRRLHTSRPYQERLLNMAEARREIVT 114
+S Q + +H ++ Q QQ Q ++Q++++ Q L + +++ +T
Sbjct: 47 SSQEQQLMQQHQQMLSYFQPQQQLLPHLQLQQQLQQQQLAQLQQQLSQLQLTLLQQQQLT 226
Query: 115 ALKFHRASMKEASEQQQQQQLQQQQQQRAPDSPQLSQNPSFDQ 157
L+ + A QQQ QLQ Q Q QL F Q
Sbjct: 227 QLQ----QQQLAQLQQQLAQLQLTQHQHTQLQQQLLPQQQFQQ 343
>BP043764
Length = 479
Score = 30.0 bits (66), Expect = 0.67
Identities = 20/65 (30%), Positives = 30/65 (45%), Gaps = 8/65 (12%)
Frame = +1
Query: 170 YPSCNTNFSSYMGDFSSYPPAFVPNSY--------PVPVASPIAPPPLMAENPNFILPNQ 221
+P+ + + S+ PP F P S P+P A+P PPPL+ P + P+Q
Sbjct: 40 FPTSHLSIPPKHHSSSATPPPFPPPSNRRRTPTTPPLPSAAPSKPPPLL---PPILNPHQ 210
Query: 222 PLGLN 226
L N
Sbjct: 211 NLNPN 225
>TC19911 weakly similar to PIR|A44805|A44805 eggshell protein precursor -
fluke (Schistosoma haematobium) {Schistosoma
haematobium;} , partial (24%)
Length = 350
Score = 29.6 bits (65), Expect = 0.87
Identities = 14/36 (38%), Positives = 16/36 (43%)
Frame = -1
Query: 187 YPPAFVPNSYPVPVASPIAPPPLMAENPNFILPNQP 222
+PP P+ YP P P PPL E P P P
Sbjct: 347 WPPERXPSPYPTPERPPSP*PPLPLERPPSPYPPPP 240
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.126 0.365
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,710,977
Number of Sequences: 28460
Number of extensions: 106522
Number of successful extensions: 1227
Number of sequences better than 10.0: 125
Number of HSP's better than 10.0 without gapping: 902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1057
length of query: 382
length of database: 4,897,600
effective HSP length: 92
effective length of query: 290
effective length of database: 2,279,280
effective search space: 660991200
effective search space used: 660991200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0133.10