BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0083.3
(287 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP053577 78 2e-15
BP071404 59 1e-09
BP080049 58 2e-09
TC10764 45 1e-05
TC13259 45 1e-05
BP075797 44 3e-05
TC19127 42 2e-04
BP032481 42 2e-04
AV780221 41 3e-04
TC17368 40 6e-04
AV767121 39 0.001
BP075268 39 0.001
TC18862 37 0.003
AV780392 34 0.003
AV768060 37 0.004
AV780729 35 0.019
TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3,... 34 0.033
TC13572 34 0.033
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 33 0.056
BP062063 32 0.096
>BP053577
Length = 463
Score = 78.2 bits (191), Expect = 2e-15
Identities = 33/71 (46%), Positives = 51/71 (71%)
Frame = +3
Query: 2 NGEILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEG 61
+GE++A+ S + +P+L+EAL LRW + +A DLGFR + +TDCL LF+ WR +EG
Sbjct: 42 DGEVMASACSSPVSISSPLLAEALGLRWTMQLATDLGFRRITLETDCLQLFNTWRSQEEG 221
Query: 62 RSHLGTVVRDC 72
RS+L +++ DC
Sbjct: 222 RSYLSSIISDC 254
>BP071404
Length = 394
Score = 58.9 bits (141), Expect = 1e-09
Identities = 23/68 (33%), Positives = 42/68 (60%)
Frame = -2
Query: 5 ILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSH 64
+L + GP L P+++EA C++W++ + R LGF+ + +TDCL+L W +S+
Sbjct: 390 LLVAASYYAGPSLDPLVAEAACMKWSMELCRMLGFQSLVLETDCLLLHQIWTARTLPKSY 211
Query: 65 LGTVVRDC 72
G+++ DC
Sbjct: 210 AGSIMEDC 187
>BP080049
Length = 356
Score = 58.2 bits (139), Expect = 2e-09
Identities = 35/89 (39%), Positives = 51/89 (56%)
Frame = -1
Query: 25 LCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSHLGTVVRDCVNWKQIVLPKNQ 84
L LRWA+G+A +LGFR + F+TDCL L++ W+R G S L T++ DC N
Sbjct: 356 LSLRWALGLASELGFR*IVFETDCLHLYEAWKRRG-GCSVLDTLILDCRNLLSYFDVFQL 180
Query: 85 GGLGVRSTRESNVSLLGKLAWKLGSRIFI 113
VR T V L KL ++LG+ +++
Sbjct: 179 S--FVRRTSNCVVDALAKLCFQLGNVVWV 99
>TC10764
Length = 555
Score = 45.4 bits (106), Expect = 1e-05
Identities = 24/70 (34%), Positives = 39/70 (55%)
Frame = +1
Query: 3 GEILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGR 62
GE+LA T++ VL P+++EA C WA+ F F+ L L++ W ++ G
Sbjct: 139 GEVLAATSAFPLSVLPPLIAEAFC--WALSSDMTSCFGSFCFEIGYLQLYETWMKT*PGS 312
Query: 63 SHLGTVVRDC 72
S++ T+V DC
Sbjct: 313 SYVCTLVNDC 342
>TC13259
Length = 506
Score = 45.1 bits (105), Expect = 1e-05
Identities = 23/74 (31%), Positives = 41/74 (55%)
Frame = +1
Query: 4 EILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRS 63
++LAT +S ++ SEA+ LRW + ++ +LGF V + D V+ + W+ + + S
Sbjct: 55 QVLATASSKWPRTVSVATSEAMALRWGLQLSLELGFFEVEVEIDNQVVVNSWKGTKKHVS 234
Query: 64 HLGTVVRDCVNWKQ 77
+L V+ D V Q
Sbjct: 235 YLANVIADVVRISQ 276
>BP075797
Length = 504
Score = 43.9 bits (102), Expect = 3e-05
Identities = 21/54 (38%), Positives = 32/54 (58%)
Frame = -2
Query: 19 PVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSHLGTVVRDC 72
P ++EAL LR I + +D FR + ++DCL L W RS +L ++V+DC
Sbjct: 407 PAIAEALSLRRTITLPQDENFRIIELESDCLQLVHAWDRSVPHPHYLASIVQDC 246
>TC19127
Length = 588
Score = 41.6 bits (96), Expect = 2e-04
Identities = 17/44 (38%), Positives = 28/44 (63%)
Frame = +1
Query: 20 VLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRS 63
+++EA+ ++WA+ R LGF + +TD LVL WR ++ G S
Sbjct: 142 IMAEAMGVQWALEWTRQLGFTQISLETDSLVLVQQWRNNNFGSS 273
>BP032481
Length = 447
Score = 41.6 bits (96), Expect = 2e-04
Identities = 16/31 (51%), Positives = 24/31 (76%)
Frame = -2
Query: 19 PVLSEALCLRWAIGMARDLGFRHVWFDTDCL 49
P+L+EA+ +RW + +A DLGFR + +TDCL
Sbjct: 353 PLLAEAMSMRWIMQLAIDLGFRRICLETDCL 261
>AV780221
Length = 461
Score = 40.8 bits (94), Expect = 3e-04
Identities = 31/110 (28%), Positives = 47/110 (42%), Gaps = 14/110 (12%)
Frame = +3
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWK-------LGSRIFITKYLKGSSIFSY 125
V W + PK++GGLGV++ N +LLGK W+ L ++ + KY
Sbjct: 153 VKWSTVCRPKDEGGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYKNA------ 314
Query: 126 ELTGSSSYVWKGLVKAF------SVLRDGLSFAIHDGC-TSFWYSDWTGL 168
+ S+S W L ++ GL I +G FW +W GL
Sbjct: 315 -IPQSASSWWNDLHSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNENWLGL 461
>TC17368
Length = 563
Score = 39.7 bits (91), Expect = 6e-04
Identities = 19/46 (41%), Positives = 24/46 (51%)
Frame = +3
Query: 26 CLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSHLGTVVRD 71
C RWAI + DLGF V F+ DC VL W +L +V+D
Sbjct: 225 CFRWAIELTMDLGFSGVIFENDC*VLHCAWISKLNSPPYLSFIVQD 362
>AV767121
Length = 525
Score = 38.9 bits (89), Expect = 0.001
Identities = 34/114 (29%), Positives = 46/114 (39%), Gaps = 13/114 (11%)
Frame = +2
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWK-------LGSRIFITK---YLKGSSI 122
V W + PK GGLG++ N +LLGK W+ L R+ + Y GSS
Sbjct: 191 VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQPDHYSCGSSW 370
Query: 123 FS--YELTGSSSYVWKGLVKAFSVLRDGLSFAIHDG-CTSFWYSDWTGLGKLCA 173
++ L + W GL + +G T FW DW G G L A
Sbjct: 371 WNDILSLCPEDADGW---------FSSGLKKLVGEGDQTKFWSEDWLGSGLLSA 505
>BP075268
Length = 437
Score = 38.9 bits (89), Expect = 0.001
Identities = 21/65 (32%), Positives = 35/65 (53%), Gaps = 5/65 (7%)
Frame = -1
Query: 13 MGPVLTPVLS----EALCLRWAIGMARDLGFRHVWFDTDCLVLFD-YWRRSDEGRSHLGT 67
+ P TP S ++ LRWA+ + DL ++ F+TDCL L W+ + S+L +
Sbjct: 323 VSPSSTPA*SYFGRSSMLLRWAMKLCMDLNLLNIIFETDCLALTTRSWKSNGGCTSYLHS 144
Query: 68 VVRDC 72
++ DC
Sbjct: 143 IMEDC 129
>TC18862
Length = 524
Score = 37.4 bits (85), Expect = 0.003
Identities = 14/42 (33%), Positives = 26/42 (61%)
Frame = +2
Query: 32 GMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSHLGTVVRDCV 73
G+ ++L F +V +T CL+L+ WR S + +++RDC+
Sbjct: 122 GLIKNLCFLNVIIETGCLILYQEWRSGRRASSCMSSMIRDCL 247
>AV780392
Length = 534
Score = 34.3 bits (77), Expect(2) = 0.003
Identities = 19/46 (41%), Positives = 24/46 (51%)
Frame = -3
Query: 120 SSIFSYELTGSSSYVWKGLVKAFSVLRDGLSFAIHDGCTSFWYSDW 165
S I S LT +S++V RDG SF + G TS WYS+W
Sbjct: 430 SGIMSSRLTFASNFV-----------RDGFSFRLQPGNTSRWYSNW 326
Score = 21.9 bits (45), Expect(2) = 0.003
Identities = 8/11 (72%), Positives = 10/11 (90%)
Frame = -2
Query: 172 CALVPFVHISD 182
C+LV +VHISD
Sbjct: 299 CSLVNYVHISD 267
>AV768060
Length = 416
Score = 37.0 bits (84), Expect = 0.004
Identities = 18/54 (33%), Positives = 30/54 (55%)
Frame = -3
Query: 19 PVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGRSHLGTVVRDC 72
P+++EA+C RWA+ +A + G + F +DC L + E S L ++ DC
Sbjct: 300 PLIAEAMCFRWALNLAANWGLDSLVFSSDCQQLVKAFHCPSEFPS-LAHIMLDC 142
>AV780729
Length = 475
Score = 34.7 bits (78), Expect = 0.019
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 4 EILATTTSVMGPVLTPVLSEALCLRWAIGMARDL 37
EILA+ T+ L+P+L+EA C RWA+ +A +L
Sbjct: 372 EILASVTAYPVTSLSPLLAEAGCFRWALRLAMEL 473
>TC11350 similar to UP|Q9LJP7 (Q9LJP7) Genomic DNA, chromosome 3, P1 clone:
MIG10, partial (18%)
Length = 1118
Score = 33.9 bits (76), Expect = 0.033
Identities = 19/54 (35%), Positives = 30/54 (55%)
Frame = -3
Query: 5 ILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRS 58
I T + + P +P++SEAL LR + +AR LG + ++DC L + R S
Sbjct: 684 ISGTASRPLAP--SPIVSEALALREGLILARSLGLDRILSESDCQPLIEACRSS 529
>TC13572
Length = 534
Score = 33.9 bits (76), Expect = 0.033
Identities = 26/101 (25%), Positives = 41/101 (39%), Gaps = 11/101 (10%)
Frame = -3
Query: 158 TSFWYSDWTGLG----KLCALVPFVHISDTALTISDVWVEGSWNLSMLY------TELPD 207
T FW +WTG+G K L + ++ W +G WN + EL
Sbjct: 334 TRFWLENWTGVGILREKYYRLYNLSKLKWASIDECGGWSQGVWNWDFRWRRPLAGRELDW 155
Query: 208 PYKPTITSMQGPSHETVDDCWVWN-SNPAGYSCSDAYQWLR 247
++ ++ P V D WVW S YS + A+ +L+
Sbjct: 154 LQALSLDIVRVPLLAGVPDKWVWKPSEDGSYSVNSAFIFLQ 32
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 33.1 bits (74), Expect = 0.056
Identities = 19/63 (30%), Positives = 28/63 (44%), Gaps = 7/63 (11%)
Frame = -1
Query: 192 VEGSWNLSMLYTELPDPYKPTITS------MQGPSHETVDDCWVWNSNPAG-YSCSDAYQ 244
VEG W S + P + + + P E +D W W +NP G Y+ AY+
Sbjct: 780 VEGVWTWSFIRRRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTANPEGQYTVQTAYK 601
Query: 245 WLR 247
+LR
Sbjct: 600 FLR 592
>BP062063
Length = 327
Score = 32.3 bits (72), Expect = 0.096
Identities = 13/31 (41%), Positives = 17/31 (53%)
Frame = -2
Query: 26 CLRWAIGMARDLGFRHVWFDTDCLVLFDYWR 56
CLRW++ + D G HV F DC L +R
Sbjct: 326 CLRWSLSVVEDWGLDHVMFSWDCQ*LVGAYR 234
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,440,206
Number of Sequences: 28460
Number of extensions: 110966
Number of successful extensions: 537
Number of sequences better than 10.0: 61
Number of HSP's better than 10.0 without gapping: 535
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 536
length of query: 287
length of database: 4,897,600
effective HSP length: 89
effective length of query: 198
effective length of database: 2,364,660
effective search space: 468202680
effective search space used: 468202680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0083.3