BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0069.5
(595 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12586 weakly similar to PIR|C86410|C86410 protein F3M18.18 [im... 155 2e-38
AV407953 101 4e-22
AV769794 99 1e-21
AV428425 97 7e-21
TC17979 82 2e-16
AV419667 82 2e-16
TC15498 weakly similar to PIR|C86410|C86410 protein F3M18.18 [im... 82 2e-16
TC15080 82 3e-16
BP084865 80 1e-15
AU252041 74 9e-14
TC17932 62 3e-10
BG662361 54 5e-08
AV410186 44 7e-05
TC14648 31 0.65
BP072007 30 1.1
TC19700 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like prot... 30 1.5
TC7952 similar to PIR|A45116|A45116 L-ascorbate peroxidase , cyt... 28 5.5
TC7951 similar to UP|Q43758 (Q43758) Ascorbate peroxidase , com... 28 5.5
AV774621 27 7.2
TC15043 weakly similar to UP|AAR87280 (AAR87280) Expressed prote... 27 7.2
>TC12586 weakly similar to PIR|C86410|C86410 protein F3M18.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(17%)
Length = 622
Score = 155 bits (391), Expect = 2e-38
Identities = 85/212 (40%), Positives = 126/212 (59%), Gaps = 7/212 (3%)
Frame = +1
Query: 175 IGGKDLVLVKQDVSSNLEPSELKKNLDELGDQLFTGTC------NFLPKTKDQKHKVPPA 228
+GG D++ KQ SS ++P E++K L ++ D F G + TK+ K +
Sbjct: 4 LGGTDIIYAKQQYSSPVQPVEVRKLLKDMADNNFLGQAGQHNAHDGKSNTKE-KFMMDNG 180
Query: 229 FDVFGPQIVAFNNSTCVCAKDGITVICAKRGGDTQVS-NHSEWLLTVPNKPDAVDFSFIP 287
D G Q ++ + I +C ++GG+ + S H++W TV ++PD + SFIP
Sbjct: 181 LDFMGIQPRSYYEAEV----QDIKFMCKRKGGNLKKSMTHNDWCQTVLSQPDLIAMSFIP 348
Query: 288 FTSLLKAAPGRGFLSHAINLYLRYKPPLSDLPYFLDYQAHRLWAPIHNDLPLGPVSNRTT 347
TSLL G G+L+HAINLYLRYKPP+ +L FL++Q R WAP+ +L LGP ++
Sbjct: 349 ITSLLGGVNGSGYLTHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFGELALGP-ERKSQ 525
Query: 348 HSPSLSLNLMGPKLYVNTAKVTVGKRPVTGMR 379
++ SL + +GPKLYVNT V VGK+PVTG+R
Sbjct: 526 NTASLQFSFLGPKLYVNTTPVDVGKKPVTGLR 621
>AV407953
Length = 416
Score = 101 bits (251), Expect = 4e-22
Identities = 63/141 (44%), Positives = 82/141 (57%), Gaps = 6/141 (4%)
Frame = +1
Query: 191 LEPSELKKNLDELGDQLFTGTCNFLPKTKDQKHKVPPAFDVFG--PQIVAFNN---STCV 245
L+P++++K L E+ D+ F DQ V P D FG Q + F N S+
Sbjct: 4 LQPADVQKRLKEMADRRFMDANGQYSVASDQ---VFPN-DKFGVREQRLTFANISPSSSY 171
Query: 246 CAKDGITVICAKRGG-DTQVSNHSEWLLTVPNKPDAVDFSFIPFTSLLKAAPGRGFLSHA 304
K+ I I +RGG D + +H EWL TV +PD + SFIP TSLL PG GFLSHA
Sbjct: 172 SHKEDIVSIYKRRGGSDDRNLSHHEWLQTVQVEPDVISMSFIPITSLLNGVPGSGFLSHA 351
Query: 305 INLYLRYKPPLSDLPYFLDYQ 325
INLYLRYKPP+ +L FL++Q
Sbjct: 352 INLYLRYKPPIEELHQFLEFQ 414
>AV769794
Length = 559
Score = 99.4 bits (246), Expect = 1e-21
Identities = 52/122 (42%), Positives = 78/122 (63%), Gaps = 2/122 (1%)
Frame = -2
Query: 473 HLRLLFSKVSNAIVVKSNWTQG-STQRSGSGSGSGSSVFSAISTSI-QGKDQKKPALVVV 530
HLRLLF+ + N + K+ W R S + S+ FS S G ++ PA++
Sbjct: 558 HLRLLFTNIPNCSIRKTEWAAAPEASRKSSFLTNLSTTFSFTQQSATSGPPKQAPAML-- 385
Query: 531 DSSVFPTGPPVPVQAQKLLKFVDTSQLCKGPQDSPGHWLVTGARLMLDKGKICLWAKFSL 590
+S ++P GPPVPV+A KLLK+V+ +++ +GP D+PGHWLVT A+L+ + GKI L KF+L
Sbjct: 384 NSGIYPDGPPVPVRAGKLLKYVEAAEVVRGPHDTPGHWLVTAAKLVTEGGKIGLQVKFAL 205
Query: 591 LN 592
L+
Sbjct: 204 LD 199
>AV428425
Length = 422
Score = 97.1 bits (240), Expect = 7e-21
Identities = 62/169 (36%), Positives = 86/169 (50%)
Frame = +3
Query: 189 SNLEPSELKKNLDELGDQLFTGTCNFLPKTKDQKHKVPPAFDVFGPQIVAFNNSTCVCAK 248
++L S ++ + E+GD F D K+K GP V + K
Sbjct: 3 ASLSASNIENYVKEIGDDRFF----------DGKNKS-------GPGPVKYKE------K 113
Query: 249 DGITVICAKRGGDTQVSNHSEWLLTVPNKPDAVDFSFIPFTSLLKAAPGRGFLSHAINLY 308
D +TVI +RGGD +H++W+ TV PD ++ F P SLL+ PG L+ AI LY
Sbjct: 114 D-VTVIFRRRGGDDLEQSHTKWVETVKLAPDIINMKFTPIASLLEGVPGVKHLARAIELY 290
Query: 309 LRYKPPLSDLPYFLDYQAHRLWAPIHNDLPLGPVSNRTTHSPSLSLNLM 357
L YKPP+ DL YFLD+Q R+WAP N+L + PSL +LM
Sbjct: 291 LEYKPPIEDLQYFLDFQITRVWAPEQNNL-----QRKEPVCPSLQFSLM 422
>TC17979
Length = 777
Score = 82.4 bits (202), Expect = 2e-16
Identities = 50/138 (36%), Positives = 75/138 (54%)
Frame = +3
Query: 455 IVTGVQLHVKKHESRSVLHLRLLFSKVSNAIVVKSNWTQGSTQRSGSGSGSGSSVFSAIS 514
+VTG QL V SR+VL+++LL+S++ + +S W Q + S +S +
Sbjct: 9 VVTGAQLGVWDFGSRNVLYMKLLYSRLPGCTIRRSLWDHIPNQPLPTVSVGNTS--NPDE 182
Query: 515 TSIQGKDQKKPALVVVDSSVFPTGPPVPVQAQKLLKFVDTSQLCKGPQDSPGHWLVTGAR 574
+SI ++ P +L+K+VD S++ KGPQD PGHWLVTG +
Sbjct: 183 SSISFRENTTPG-------------------NELVKYVDLSEMSKGPQDPPGHWLVTGGK 305
Query: 575 LMLDKGKICLWAKFSLLN 592
L ++KGKI L K+SLLN
Sbjct: 306 LGVEKGKIVLRLKYSLLN 359
>AV419667
Length = 406
Score = 82.0 bits (201), Expect = 2e-16
Identities = 54/127 (42%), Positives = 74/127 (57%), Gaps = 2/127 (1%)
Frame = +1
Query: 360 KLYVNTAKVTVGKRPVTGMRLFLEGMKCNRLAIHLQYLLNTPT--MLNNKIEDTTTWSEE 417
+L +N +V VGK+PVTG+RL+LEG K N LAIHLQ+L + P L ++ S E
Sbjct: 46 RLTLNYLQVDVGKKPVTGLRLYLEGKKNNCLAIHLQHLSSLPKTFQLMDEPNGNVPHSSE 225
Query: 418 IIDDRFLEAISGKKFSHVCTAPVKYNPSWSSDKDVAFIVTGVQLHVKKHESRSVLHLRLL 477
++ E + K FSHVCTAPV+ + S +VTG + V + + VL LRL
Sbjct: 226 ---RKYYEKVQWKSFSHVCTAPVESDGENS-------VVTGARF*VGESGLKKVLFLRLH 375
Query: 478 FSKVSNA 484
FSKV+ A
Sbjct: 376 FSKVAGA 396
>TC15498 weakly similar to PIR|C86410|C86410 protein F3M18.18 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(7%)
Length = 515
Score = 82.0 bits (201), Expect = 2e-16
Identities = 53/126 (42%), Positives = 71/126 (56%), Gaps = 1/126 (0%)
Frame = +3
Query: 468 SRSVLHLRLLFSKVSNAIVVKSNWTQG-STQRSGSGSGSGSSVFSAISTSIQGKDQKKPA 526
+++VLHL+LLFSKV + +S W ST + + G+S TS D KK
Sbjct: 15 AQNVLHLKLLFSKVPGCTIRRSVWDHNPSTPATAAQRSDGASSSLTKKTS----DDKKE- 179
Query: 527 LVVVDSSVFPTGPPVPVQAQKLLKFVDTSQLCKGPQDSPGHWLVTGARLMLDKGKICLWA 586
DSS+ KL K VD +++ +GPQD PGHWLVTGA+L ++KGKI L
Sbjct: 180 ----DSSI---------HIGKLAKIVDMTEMFRGPQDIPGHWLVTGAKLGVEKGKIVLRI 320
Query: 587 KFSLLN 592
K+SLLN
Sbjct: 321 KYSLLN 338
>TC15080
Length = 716
Score = 81.6 bits (200), Expect = 3e-16
Identities = 35/59 (59%), Positives = 50/59 (84%)
Frame = +1
Query: 532 SSVFPTGPPVPVQAQKLLKFVDTSQLCKGPQDSPGHWLVTGARLMLDKGKICLWAKFSL 590
S+++P GPPV Q+ KLL+FVDT+++ +GPQDSPG+W+V+GARL ++KGKI L K+SL
Sbjct: 1 SALYPGGPPVXAQSTKLLRFVDTTEMTRGPQDSPGYWVVSGARLYVEKGKISLKVKYSL 177
>BP084865
Length = 484
Score = 79.7 bits (195), Expect = 1e-15
Identities = 37/82 (45%), Positives = 57/82 (69%), Gaps = 3/82 (3%)
Frame = -3
Query: 514 STSIQGKDQK---KPALVVVDSSVFPTGPPVPVQAQKLLKFVDTSQLCKGPQDSPGHWLV 570
++S+ K++K K V + S+ +PTGPP VQ LL+FVDT+++ +GP+ +PG+W+V
Sbjct: 428 ASSVHEKEKKYTPKGGEVTIGSAAYPTGPPSRVQTPSLLRFVDTAEIIRGPEHTPGYWVV 249
Query: 571 TGARLMLDKGKICLWAKFSLLN 592
+GAR + GKI L K+SLLN
Sbjct: 248 SGARFSVQNGKIYLSVKYSLLN 183
>AU252041
Length = 326
Score = 73.6 bits (179), Expect = 9e-14
Identities = 39/94 (41%), Positives = 58/94 (61%), Gaps = 2/94 (2%)
Frame = +3
Query: 9 ALNSLGKGFDLTSDFRLKFCKGE--ERLVILNETERRELTVPGFGPVTDVSVDIKCDKGD 66
++ +LG+GFD+TSD RL +CKG RLV +E R+L + V +VS DI C G
Sbjct: 45 SIQALGRGFDVTSDIRLLYCKGAPGSRLVRFDEEHTRDLAISHGVVVPNVSGDIVCSLGK 224
Query: 67 LTRYQSDILSFTQMSEMFNQKSSIPGKIPSGYFN 100
++ + SF +M++ FN++S I G+IP G FN
Sbjct: 225 SALEKTPVCSFHEMAKHFNERSGITGQIPLGSFN 326
>TC17932
Length = 572
Score = 61.6 bits (148), Expect = 3e-10
Identities = 24/43 (55%), Positives = 39/43 (89%)
Frame = +1
Query: 549 LKFVDTSQLCKGPQDSPGHWLVTGARLMLDKGKICLWAKFSLL 591
LKFVDT+++ +GPQ++PG+W+V+GARL+++KG++ L K+SLL
Sbjct: 1 LKFVDTTEMSRGPQENPGYWVVSGARLVVEKGRVSLRVKYSLL 129
>BG662361
Length = 330
Score = 54.3 bits (129), Expect = 5e-08
Identities = 33/77 (42%), Positives = 45/77 (57%), Gaps = 1/77 (1%)
Frame = +1
Query: 307 LYLRYKPPLSDLPYFLDYQAHRLWAPIHNDLPLGPVSNRTTHSPSLSLNLMGPKLYVNTA 366
LY KPP+ DL YFLD+Q R+WAP N+L PS+ L+ G + +
Sbjct: 115 LYEPDKPPIEDLQYFLDFQITRVWAPEQNNL---QTKGACLSIPSVQLD--GT*AFCSVP 279
Query: 367 -KVTVGKRPVTGMRLFL 382
+VTVG++PVTG+RL L
Sbjct: 280 DQVTVGRKPVTGLRLSL 330
>AV410186
Length = 433
Score = 43.9 bits (102), Expect = 7e-05
Identities = 21/28 (75%), Positives = 22/28 (78%)
Frame = +2
Query: 3 EGIVEKALNSLGKGFDLTSDFRLKFCKG 30
EGI AL SLGKGFDL SDFRL+F KG
Sbjct: 323 EGIEMVALESLGKGFDLASDFRLRFAKG 406
>TC14648
Length = 669
Score = 30.8 bits (68), Expect = 0.65
Identities = 22/67 (32%), Positives = 32/67 (46%)
Frame = -2
Query: 258 RGGDTQVSNHSEWLLTVPNKPDAVDFSFIPFTSLLKAAPGRGFLSHAINLYLRYKPPLSD 317
RGG QV +H W++ VP+ +A I SL++A L H ++L L + D
Sbjct: 269 RGGVEQVLSHFHWVILVPSLVEAQLLPHILVPSLVEAQ----LLPHILSLLLHWVSSPQD 102
Query: 318 LPYFLDY 324
P L Y
Sbjct: 101 -PSLLSY 84
>BP072007
Length = 362
Score = 30.0 bits (66), Expect = 1.1
Identities = 16/26 (61%), Positives = 19/26 (72%), Gaps = 1/26 (3%)
Frame = -3
Query: 568 WLVT-GARLMLDKGKICLWAKFSLLN 592
WLVT G L ++KGKI L K+SLLN
Sbjct: 234 WLVTTGLELGVEKGKIVLRIKYSLLN 157
>TC19700 similar to UP|Q9SVU5 (Q9SVU5) Proline-rich APG-like protein,
partial (25%)
Length = 442
Score = 29.6 bits (65), Expect = 1.5
Identities = 35/120 (29%), Positives = 47/120 (39%), Gaps = 17/120 (14%)
Frame = +1
Query: 276 NKPDAVDFS----FIPFTSLLKAAPGRGF-------LSHAINLYLRYKPPLSDLPYFLDY 324
+ P +V FS I ++ KAA GR F + + N YL PY +DY
Sbjct: 82 HNPCSVFFSSCLVIIMLINIPKAAEGRAFFVFGDSLVDNGNNNYLITTARADSYPYGIDY 261
Query: 325 QAHRLWAPIHNDLPLGPVSNRTTHS----PSLSLNLMGPKLYV--NTAKVTVGKRPVTGM 378
HR N L + + + S P LS L G L V N A +G TG+
Sbjct: 262 PTHRATGRFSNGLNIPDLISERIGSEPTLPYLSPQLDGEALLVGANFASAGIGILNDTGI 441
>TC7952 similar to PIR|A45116|A45116 L-ascorbate peroxidase , cytosolic
[validated] - garden pea {Pisum sativum;}
, partial (62%)
Length = 542
Score = 27.7 bits (60), Expect = 5.5
Identities = 19/68 (27%), Positives = 32/68 (46%)
Frame = -3
Query: 481 VSNAIVVKSNWTQGSTQRSGSGSGSGSSVFSAISTSIQGKDQKKPALVVVDSSVFPTGPP 540
V++ +NW + + + G+ S SGS+ +A+S + A +V P GPP
Sbjct: 396 VTSTATTPANW*KSA*DKMGNCSLSGSNSLTAMSRPLLAP*ASSAACFMV-----PKGPP 232
Query: 541 VPVQAQKL 548
V V K+
Sbjct: 231 VLVFTSKV 208
>TC7951 similar to UP|Q43758 (Q43758) Ascorbate peroxidase , complete
Length = 1022
Score = 27.7 bits (60), Expect = 5.5
Identities = 19/68 (27%), Positives = 32/68 (46%)
Frame = -3
Query: 481 VSNAIVVKSNWTQGSTQRSGSGSGSGSSVFSAISTSIQGKDQKKPALVVVDSSVFPTGPP 540
V++ +NW + + + G+ S SGS+ +A+S + A +V P GPP
Sbjct: 390 VTSTATTPANW*KSA*DKMGNCSLSGSNSLTAMSRPLLAP*ASSAACFMV-----PKGPP 226
Query: 541 VPVQAQKL 548
V V K+
Sbjct: 225 VLVFTSKV 202
>AV774621
Length = 356
Score = 27.3 bits (59), Expect = 7.2
Identities = 14/43 (32%), Positives = 23/43 (52%)
Frame = +1
Query: 495 STQRSGSGSGSGSSVFSAISTSIQGKDQKKPALVVVDSSVFPT 537
+TQ G SGSG+S FS + T + + L ++ ++PT
Sbjct: 85 NTQHVGMRSGSGTSGFSHLLTGTRPLSPE*DCLSILSYPIYPT 213
>TC15043 weakly similar to UP|AAR87280 (AAR87280) Expressed protein (With
alternative splicing), partial (10%)
Length = 1269
Score = 27.3 bits (59), Expect = 7.2
Identities = 12/35 (34%), Positives = 20/35 (56%)
Frame = +2
Query: 139 LVLSKQVIEAVPSSWDPTALARFIEKFGTHILVGL 173
+V+ K + A SWDPT++ F+ + IL G+
Sbjct: 749 VVVPKTRVHASWLSWDPTSIPLFVSLLSSSILCGV 853
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,472,311
Number of Sequences: 28460
Number of extensions: 149981
Number of successful extensions: 767
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 759
length of query: 595
length of database: 4,897,600
effective HSP length: 95
effective length of query: 500
effective length of database: 2,193,900
effective search space: 1096950000
effective search space used: 1096950000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0069.5