BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0034a.1
(384 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19125 UP|GBA1_LOTJA (P49082) Guanine nucleotide-binding protei... 772 0.0
TC9784 weakly similar to UP|Q9LI02 (Q9LI02) ESTs C74776(E51022),... 115 9e-27
CB828443 58 3e-09
TC15063 homologue to UP|SARA_ARATH (O04834) GTP-binding protein ... 40 9e-04
TC12986 homologue to UP|SARB_ARATH (Q01474) GTP-binding protein ... 38 0.002
TC14160 homologue to GB|BAB90396.1|20161472|AP003570 ADP-ribosyl... 38 0.003
TC10350 homologue to UP|AAR29293 (AAR29293) ADP-ribosylation fac... 38 0.003
TC14161 UP|AAR29293 (AAR29293) ADP-ribosylation factor, complete 38 0.003
AV426265 37 0.006
TC15817 32 0.23
TC17859 homologue to UP|Q8VY57 (Q8VY57) ADP-ribosylation factor-... 30 0.88
TC8016 homologue to UP|CAT4_SOYBN (O48561) Catalase 4 , partial... 29 1.1
TC16963 28 3.3
TC11621 27 4.4
AV780046 26 9.7
TC9634 similar to UP|Q8W0Z3 (Q8W0Z3) At1g29350/F15D2_27, partial... 26 9.7
>TC19125 UP|GBA1_LOTJA (P49082) Guanine nucleotide-binding protein alpha-1
subunit (GP-alpha-1), complete
Length = 1597
Score = 772 bits (1994), Expect = 0.0
Identities = 383/384 (99%), Positives = 383/384 (99%)
Frame = +2
Query: 1 MGLLCSKNRRYNDADTEENAQTAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIK 60
MGLLCSKNRRYNDADTEEN QTAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIK
Sbjct: 11 MGLLCSKNRRYNDADTEENTQTAEIERRIELETKAEKHIQKLLLLGAGESGKSTIFKQIK 190
Query: 61 LLFQTGFDEAELKSYQPVIHANVYQTIKLLHDGAKELAQNDVDFSKYVISDENKEIGEKL 120
LLFQTGFDEAELKSYQPVIHANVYQTIKLLHDGAKELAQNDVDFSKYVISDENKEIGEKL
Sbjct: 191 LLFQTGFDEAELKSYQPVIHANVYQTIKLLHDGAKELAQNDVDFSKYVISDENKEIGEKL 370
Query: 121 SEIGGRLDYPCLTKELALEIENLWKDAAIQETYARGNELQVPDCTHYFMENLHRLSDANY 180
SEIGGRLDYPCLTKELALEIENLWKDAAIQETYARGNELQVPDCTHYFMENLHRLSDANY
Sbjct: 371 SEIGGRLDYPCLTKELALEIENLWKDAAIQETYARGNELQVPDCTHYFMENLHRLSDANY 550
Query: 181 VPTKDDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVSAV 240
VPTKDDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVSAV
Sbjct: 551 VPTKDDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRKWIHLFEGVSAV 730
Query: 241 IFCAAISEYDQTLFEDENKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDIFEKKILKV 300
IFCAAISEYDQTLFEDENKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDIFEKKILKV
Sbjct: 731 IFCAAISEYDQTLFEDENKNRMMETKELFEWVLKQPCFEKTSFMLFLNKFDIFEKKILKV 910
Query: 301 PLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQNTAPDRVDRVFKIYRTTALDQK 360
PLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQNTAPDRVDRVFKIYRTTALDQK
Sbjct: 911 PLNVCEWFKDYQPVSTGKQEIEHAYEFVKKKFEESYFQNTAPDRVDRVFKIYRTTALDQK 1090
Query: 361 VVKKTFKLVDETLRRRNLFEAGLL 384
VVKKTFKLVDETLRRRNLFEAGLL
Sbjct: 1091VVKKTFKLVDETLRRRNLFEAGLL 1162
>TC9784 weakly similar to UP|Q9LI02 (Q9LI02) ESTs C74776(E51022), partial
(22%)
Length = 812
Score = 115 bits (289), Expect = 9e-27
Identities = 63/171 (36%), Positives = 101/171 (58%), Gaps = 11/171 (6%)
Frame = +1
Query: 215 YRLFDVGGQ-RNERRKWIHLFEGVSAVIFCAAISEYDQTLFEDEN-------KNRMMETK 266
Y+L V + +E KW+ +FE V AV+FC ++S++DQ ++ +N+M+++K
Sbjct: 7 YQLIRVNAKGMSEGCKWVEMFEDVRAVVFCVSLSDFDQLWLAPDSSGSGTLLQNKMIQSK 186
Query: 267 ELFEWVLKQPCFEKTSFMLFLNKFDIFEKKILKVPLNVCEWFKDYQPV--STGKQEIEH- 323
ELFE +++ PCF+ T F+L LNK+D+FE+K+ +V LN CEWF D+ PV Q + H
Sbjct: 187 ELFETMVRHPCFKDTPFVLVLNKYDMFEEKMSRVSLNACEWFSDFCPVLAHQNNQSLAHQ 366
Query: 324 AYEFVKKKFEESYFQNTAPDRVDRVFKIYRTTALDQKVVKKTFKLVDETLR 374
AY +V KF++ Y T R + + A D+ V + FK + E L+
Sbjct: 367 AYFYVAMKFKDLYASLTG-----RKLFVAQARARDRVTVDEAFKYIKEVLK 504
>CB828443
Length = 545
Score = 57.8 bits (138), Expect = 3e-09
Identities = 43/135 (31%), Positives = 65/135 (47%), Gaps = 26/135 (19%)
Frame = +3
Query: 39 IQKLLLLGAGESGKSTIFKQIKLLFQT-GFDEAELKSYQPVIHANVYQTIKLLHDGAKEL 97
+QKLLL+G SG STIFKQ K+L+++ F E E ++ + I +NVY + +L +G +
Sbjct: 141 VQKLLLVGCSGSGTSTIFKQAKILYKSIPFSEEERENIKLTIQSNVYAYLGILLEGREHF 320
Query: 98 AQNDV---------------------DFSKYVISDENKEIGEKL--SEIGGRLD--YPCL 132
+ D + Y I K + L + + G+LD +P
Sbjct: 321 EDESLADLKKRKSSELDTTGTSAKLDDKTVYSIGARLKAFSDWLLKTMVSGKLDAIFPAA 500
Query: 133 TKELALEIENLWKDA 147
T+E A IE LW DA
Sbjct: 501 TREYAPLIEELWNDA 545
>TC15063 homologue to UP|SARA_ARATH (O04834) GTP-binding protein SAR1A,
complete
Length = 933
Score = 39.7 bits (91), Expect = 9e-04
Identities = 25/90 (27%), Positives = 41/90 (44%), Gaps = 1/90 (1%)
Frame = +2
Query: 204 PVGENKKSGEV-YRLFDVGGQRNERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRM 262
P E G++ ++ FD+GG + RR W + V AV++ + YD K R
Sbjct: 326 PTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVY--LVDAYD--------KERF 475
Query: 263 METKELFEWVLKQPCFEKTSFMLFLNKFDI 292
E+K+ + +L F++ NK DI
Sbjct: 476 AESKKELDALLSDESLANVPFLILGNKIDI 565
>TC12986 homologue to UP|SARB_ARATH (Q01474) GTP-binding protein SAR1B,
partial (80%)
Length = 515
Score = 38.1 bits (87), Expect = 0.002
Identities = 25/90 (27%), Positives = 40/90 (43%), Gaps = 1/90 (1%)
Frame = +1
Query: 204 PVGENKKSGEV-YRLFDVGGQRNERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRM 262
P E G++ ++ FD+GG + RR W + V AV + + YD K R
Sbjct: 211 PTSEELSIGKIKFKAFDLGGHQVARRVWKDYYAKVDAVAY--LVDAYD--------KERF 360
Query: 263 METKELFEWVLKQPCFEKTSFMLFLNKFDI 292
E+K+ + +L F++ NK DI
Sbjct: 361 AESKKELDALLSDEALASVPFLVLGNKIDI 450
>TC14160 homologue to GB|BAB90396.1|20161472|AP003570 ADP-ribosylation
factor {Oryza sativa (japonica cultivar-group);},
partial (48%)
Length = 1105
Score = 37.7 bits (86), Expect = 0.003
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +3
Query: 215 YRLFDVGGQRNERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRMMETKELFEWVLK 274
+ ++DVGGQ R W H F+ +IF ++ D+ + + +RM+ EL + VL
Sbjct: 393 FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVL- 569
Query: 275 QPCFEKTSFMLFLNKFDI 292
++F NK D+
Sbjct: 570 ---------LVFANKQDL 596
>TC10350 homologue to UP|AAR29293 (AAR29293) ADP-ribosylation factor,
partial (98%)
Length = 603
Score = 37.7 bits (86), Expect = 0.003
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +3
Query: 215 YRLFDVGGQRNERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRMMETKELFEWVLK 274
+ ++DVGGQ R W H F+ +IF ++ D+ + + +RM+ EL + VL
Sbjct: 258 FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRAVEARDELHRMLNEDELRDAVL- 434
Query: 275 QPCFEKTSFMLFLNKFDI 292
++F NK D+
Sbjct: 435 ---------LVFANKQDL 461
>TC14161 UP|AAR29293 (AAR29293) ADP-ribosylation factor, complete
Length = 912
Score = 37.7 bits (86), Expect = 0.003
Identities = 22/78 (28%), Positives = 38/78 (48%)
Frame = +2
Query: 215 YRLFDVGGQRNERRKWIHLFEGVSAVIFCAAISEYDQTLFEDENKNRMMETKELFEWVLK 274
+ ++DVGGQ R W H F+ +IF ++ D+ + + +RM+ EL + VL
Sbjct: 275 FTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVL- 451
Query: 275 QPCFEKTSFMLFLNKFDI 292
++F NK D+
Sbjct: 452 ---------LVFANKQDL 478
>AV426265
Length = 293
Score = 37.0 bits (84), Expect = 0.006
Identities = 27/82 (32%), Positives = 43/82 (51%), Gaps = 16/82 (19%)
Frame = +2
Query: 186 DVLYAR--VRTTGVVEIQFS-----PVGENKKSGEV--------YRLFDVGGQR-NERRK 229
DVLYA ++ G+ ++FS P+ E SG + Y+L + + + K
Sbjct: 11 DVLYAEGVAQSNGLAFMEFSFDDRSPMSEIY-SGNISSQTPLIKYQLIRINSKGLRDGCK 187
Query: 230 WIHLFEGVSAVIFCAAISEYDQ 251
W+ +FE V VIFC ++S+YDQ
Sbjct: 188 WLQMFEDVRIVIFCVSLSDYDQ 253
>TC15817
Length = 773
Score = 31.6 bits (70), Expect = 0.23
Identities = 18/57 (31%), Positives = 32/57 (55%)
Frame = +3
Query: 173 HRLSDANYVPTKDDVLYARVRTTGVVEIQFSPVGENKKSGEVYRLFDVGGQRNERRK 229
H+LS+ YV + + R T V + ++ G+ KKSGE+ ++FD+ ++ RK
Sbjct: 312 HQLSNGLYVSGRPEQPKERTPTMSSVAMPYTG-GDIKKSGELGKMFDIPSDGSKSRK 479
>TC17859 homologue to UP|Q8VY57 (Q8VY57) ADP-ribosylation factor-like
protein, partial (71%)
Length = 445
Score = 29.6 bits (65), Expect = 0.88
Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 4/63 (6%)
Frame = +3
Query: 192 VRTTGVVEIQFSPVGENK----KSGEVYRLFDVGGQRNERRKWIHLFEGVSAVIFCAAIS 247
V T G E VG N K +L+D+GGQ R W VSA+++ +
Sbjct: 168 VATGGYSEDMIPTVGFNMRKVTKGNVTIKLWDLGGQPRFRSMWERYCRAVSAIVYVVDAA 347
Query: 248 EYD 250
+ D
Sbjct: 348 DPD 356
>TC8016 homologue to UP|CAT4_SOYBN (O48561) Catalase 4 , partial (28%)
Length = 727
Score = 29.3 bits (64), Expect = 1.1
Identities = 23/84 (27%), Positives = 33/84 (38%), Gaps = 14/84 (16%)
Frame = +1
Query: 162 PDCTHY------FMENLHRLSDANYVPTK-DDVLYAR-------VRTTGVVEIQFSPVGE 207
P C H+ FM +HR + NY P++ D V +A + T
Sbjct: 37 PKCAHHNNHHEGFMNFMHRDEEVNYFPSRYDPVRHAERYPIPPAICTGSRERCAIEKENN 216
Query: 208 NKKSGEVYRLFDVGGQRNERRKWI 231
K+ GE YR F Q R+W+
Sbjct: 217 FKQPGERYRSFAPDRQDRFVRRWV 288
>TC16963
Length = 532
Score = 27.7 bits (60), Expect = 3.3
Identities = 13/37 (35%), Positives = 20/37 (53%), Gaps = 4/37 (10%)
Frame = -3
Query: 269 FEWVLKQPCFEKTSFMLFLNKFDIF----EKKILKVP 301
F WV+ +EK+ + LNK+ I K I+K+P
Sbjct: 221 FHWVMAHVIYEKSGSSITLNKYKIICTTTSKNIIKLP 111
>TC11621
Length = 430
Score = 27.3 bits (59), Expect = 4.4
Identities = 9/18 (50%), Positives = 15/18 (83%)
Frame = +2
Query: 102 VDFSKYVISDENKEIGEK 119
+DF+ ++ SDEN E+GE+
Sbjct: 335 LDFNNFLCSDENNEVGER 388
>AV780046
Length = 570
Score = 26.2 bits (56), Expect = 9.7
Identities = 10/27 (37%), Positives = 20/27 (74%)
Frame = +1
Query: 164 CTHYFMENLHRLSDANYVPTKDDVLYA 190
C+HYF +N LSD+ +VP++ ++++
Sbjct: 331 CSHYF-KNRDTLSDSGFVPSRGALVHS 408
>TC9634 similar to UP|Q8W0Z3 (Q8W0Z3) At1g29350/F15D2_27, partial (7%)
Length = 687
Score = 26.2 bits (56), Expect = 9.7
Identities = 14/53 (26%), Positives = 25/53 (46%)
Frame = +2
Query: 153 YARGNELQVPDCTHYFMENLHRLSDANYVPTKDDVLYARVRTTGVVEIQFSPV 205
+ G +L + D T YF++ L+ L P + ++ AR T +SP+
Sbjct: 392 FESGVDLVIGDVTDYFLQALNHLPTRRRDPRE*SLVVARSATDFAAHGGYSPI 550
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.136 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,516,276
Number of Sequences: 28460
Number of extensions: 63370
Number of successful extensions: 317
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 316
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 316
length of query: 384
length of database: 4,897,600
effective HSP length: 92
effective length of query: 292
effective length of database: 2,279,280
effective search space: 665549760
effective search space used: 665549760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Lotus: description of TM0034a.1