BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0022.14
(334 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BU494263 68 2e-12
BP033777 62 1e-10
AV769506 61 2e-10
NP459593 Pge1 protein [Lotus japonicus] 60 7e-10
TC12588 54 4e-08
CB828260 54 4e-08
BP069013 38 0.002
TC14416 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, parti... 33 0.051
AV426322 33 0.051
TC8302 32 0.15
BP042651 32 0.20
TC10495 31 0.33
AV424663 30 0.57
TC9202 similar to UP|Q93Z17 (Q93Z17) AT5g46160/MCL19_22 (HUELLEN... 29 1.3
BI418488 28 2.2
AV775734 28 2.2
TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-... 27 3.7
TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide su... 27 3.7
BP028931 27 4.8
TC19822 similar to UP|EX13_ARATH (Q9M9P0) Alpha-expansin 13 prec... 27 4.8
>BU494263
Length = 420
Score = 67.8 bits (164), Expect = 2e-12
Identities = 48/116 (41%), Positives = 52/116 (44%), Gaps = 51/116 (43%)
Frame = -3
Query: 262 VRQSTHRRREGQLQRII------------------------------------------- 278
VRQSTHRRREGQLQRI+
Sbjct: 355 VRQSTHRRREGQLQRIM*IITSIDTDNRIAT*GL*LGITS*GYIFHNEHK*Q*KTLNMFQ 176
Query: 279 --LTSINHTRQLSQYACCMN------EMPVDKMHSGGMGINGSALTPATVGPFRPA 326
LT+ NH RQLSQYACCM P+ + M INGSAL PATVGPFRPA
Sbjct: 175 I*LTNNNHIRQLSQYACCMK*YAG*PSQPI--CNPERMDINGSALAPATVGPFRPA 14
Score = 40.0 bits (92), Expect = 5e-04
Identities = 18/32 (56%), Positives = 21/32 (65%)
Frame = -2
Query: 241 SSPRRVNHREPVRHGHLTMGVVRQSTHRRREG 272
SSPRRVNH + VRHGHLT GV ++ G
Sbjct: 419 SSPRRVNHHDLVRHGHLTKGVCSPKHTQKTRG 324
>BP033777
Length = 576
Score = 62.4 bits (150), Expect = 1e-10
Identities = 51/144 (35%), Positives = 58/144 (39%), Gaps = 49/144 (34%)
Frame = +3
Query: 236 RHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQ---------------------- 273
+H SS PRRVN RE VR HLT GV ++ G
Sbjct: 105 QHPNSSFPRRVNRRELVRQRHLTKGVCSPKHTQKTRGSTPKNYVNNSKDRYR**DSHLGF 284
Query: 274 -----------------------LQRIILTSINHTRQLSQYACCMNEM---PVDKMHSG- 306
+ +I LTSI HT N+M PV+ MHSG
Sbjct: 285 *LGITS*GCISHDEYKRR*ITVNMPQIELTSIKHTHH*VSMHVA*NDMQVNPVNHMHSGK 464
Query: 307 GMGINGSALTPATVGPFRPACLLH 330
G N SALTPATV PF PACLLH
Sbjct: 465 GWTSNRSALTPATVEPFWPACLLH 536
>AV769506
Length = 345
Score = 61.2 bits (147), Expect = 2e-10
Identities = 31/93 (33%), Positives = 54/93 (57%), Gaps = 8/93 (8%)
Frame = +1
Query: 44 KVFASRDEILEWARNLGKQHGFIIVITRSDNGGLK-RKTFMILGCERCDKYVPYK----- 97
++F +RD+++ W + ++G++ +IT+SD GG + RK +++LGCE+ KYVPY+
Sbjct: 37 QIFPTRDDLINWVHGIAIENGYVTLITKSDYGGNESRKAYVMLGCEKHGKYVPYRDPDLV 216
Query: 98 --EVLKHQSTGTKKCYCPFRLRARGTKSSTNMF 128
E QST C C +G+K S ++
Sbjct: 217 ADEDPSAQSTLEPACDCLNHHHKQGSKPSPQLW 315
>NP459593 Pge1 protein [Lotus japonicus]
Length = 633
Score = 59.7 bits (143), Expect = 7e-10
Identities = 23/50 (46%), Positives = 37/50 (74%)
Frame = +1
Query: 44 KVFASRDEILEWARNLGKQHGFIIVITRSDNGGLKRKTFMILGCERCDKY 93
+ FAS ++++WAR +GK++G+++++ RSD G KRK + LGCER KY
Sbjct: 463 EAFASHTDLIDWARCVGKENGYVVIVIRSDYGSAKRKPLITLGCERGGKY 612
>TC12588
Length = 878
Score = 53.9 bits (128), Expect = 4e-08
Identities = 50/179 (27%), Positives = 68/179 (37%), Gaps = 60/179 (33%)
Frame = -3
Query: 177 DQSHSFGGVTLVTKKEIKRKNSYDSTPTLILSRGAQSVSPQHSFRQVSQTVD-----TVL 231
+ + S+ T + K+ S DSTPTLI SRGA + + + S + +
Sbjct: 609 NHTKSYRWETTLFPKKTNDVQS*DSTPTLIPSRGAHTSTKTYMLA*SSPESEIQNDLVYV 430
Query: 232 HNPPR----------HLESSSPRRVNHREPVRHGHLTMGV-----------------VRQ 264
H+P HL SS RR+NH E R HLT GV V
Sbjct: 429 HHPSSSRNRDTLQFPHLNPSSSRRMNHHESARQRHLTKGVCSPKHTQKTRGSTPRNYVNN 250
Query: 265 STHRRR----------------------------EGQLQRIILTSINHTRQLSQYACCM 295
ST++ + + ++ LTSI HT QL QYACCM
Sbjct: 249 STNKYK*DNSHLGL*LGITS*GCIFPQRI*RL*ITVNMHQVELTSIKHTHQLCQYACCM 73
>CB828260
Length = 557
Score = 53.9 bits (128), Expect = 4e-08
Identities = 26/56 (46%), Positives = 38/56 (67%), Gaps = 1/56 (1%)
Frame = +3
Query: 72 SDNGGL-KRKTFMILGCERCDKYVPYKEVLKHQSTGTKKCYCPFRLRARGTKSSTN 126
SD+GG RK +++LGCE+ KYVPY++ + T T+K CPFRL+ R K+ T+
Sbjct: 150 SDSGGNGSRKAYVMLGCEKHGKYVPYRDPDLVEGTRTQKTECPFRLKGRPMKNGTD 317
>BP069013
Length = 470
Score = 38.1 bits (87), Expect = 0.002
Identities = 28/60 (46%), Positives = 34/60 (56%)
Frame = +3
Query: 198 SYDSTPTLILSRGAQSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVRHGHL 257
S D TPTLI SR ++SV+ V Q VL + L+ + P RVNHREPVRH L
Sbjct: 297 SIDPTPTLIPSRVSRSVASG-----VDQP*TVVLLI--KLLQPTFP*RVNHREPVRHSQL 455
>TC14416 similar to UP|Q8SMH8 (Q8SMH8) RNA-binding protein, partial (28%)
Length = 695
Score = 33.5 bits (75), Expect = 0.051
Identities = 44/147 (29%), Positives = 61/147 (40%), Gaps = 12/147 (8%)
Frame = +3
Query: 142 PPPMGSTVHAITSTSFFDFFYYPLDAFSSKQLMTPDQSHSFGGV--TLVTKKEIKRKNSY 199
P P +H+ T SFF FFY+ F L+ H+ GG+ +L T + ++N
Sbjct: 33 PFPNPFFIHSSTLLSFFFFFYF----FHVYHLL--QIPHNNGGIMSSLCTLLTLLQQN-- 188
Query: 200 DSTPTLILSRGAQSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVN----HREPVRHG 255
TP L+ S Q QT T++H P S PRR N R P+R
Sbjct: 189 -QTPLLL------------SSLQTPQT-PTLIHQP---FTRSLPRRHNPPPLFRAPLRRP 317
Query: 256 HLTMGVVRQSTH------RRREGQLQR 276
+G R+ H RRR + QR
Sbjct: 318 DFRLGPARRRQHAHPRRPRRRSRRCQR 398
>AV426322
Length = 323
Score = 33.5 bits (75), Expect = 0.051
Identities = 16/23 (69%), Positives = 19/23 (82%)
Frame = +1
Query: 1 MSPMKSSEVDKPEEKPLTGKLVR 23
MSP+KS EVDK EEK L+ KLV+
Sbjct: 253 MSPVKSYEVDKSEEKSLSQKLVK 321
>TC8302
Length = 494
Score = 32.0 bits (71), Expect = 0.15
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = +1
Query: 182 FGGVTLVTKKEIKRKN 197
FGGVTLVT+K IKRKN
Sbjct: 430 FGGVTLVTQKRIKRKN 477
>BP042651
Length = 534
Score = 31.6 bits (70), Expect = 0.20
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +3
Query: 132 PISLVITLDSPPPMGSTVHAITSTSFFDFFYYPLDAFSSKQLMTPDQSHS 181
P++ ++ SPPP S + TS S F F P+D + Q M ++H+
Sbjct: 93 PLTYTHSIPSPPPPTSLIEQATSPSHFSTFPIPIDR-RNHQTMMDSENHT 239
>TC10495
Length = 909
Score = 30.8 bits (68), Expect = 0.33
Identities = 19/47 (40%), Positives = 22/47 (46%)
Frame = +1
Query: 201 STPTLILSRGAQSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVN 247
STP LI+ Q QH R+ S T NPP HLE S +N
Sbjct: 127 STPNLIMHHHHQKRQRQHHRRKKSTT------NPPNHLERSGSGALN 249
>AV424663
Length = 427
Score = 30.0 bits (66), Expect = 0.57
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = +2
Query: 276 RIILTSINHTRQLSQYACCM 295
+I LT+ NH RQL+ YACCM
Sbjct: 50 QIGLTNNNHIRQLN*YACCM 109
>TC9202 similar to UP|Q93Z17 (Q93Z17) AT5g46160/MCL19_22 (HUELLENLOS
PARALOG), partial (71%)
Length = 872
Score = 28.9 bits (63), Expect = 1.3
Identities = 23/82 (28%), Positives = 37/82 (45%), Gaps = 11/82 (13%)
Frame = -2
Query: 112 CPFRLRARGTKSSTNM------FTKQPISLVITLDSPP---PMGSTVHAITSTSFFDFFY 162
C FRL + GT T + + + +L L SPP P G T + F FF
Sbjct: 640 CFFRLSS*GTGPKTLVPIGWPCLSTRTTALSSNLTSPPSHLPFGMAARTTTPYTTFPFFT 461
Query: 163 YPLD--AFSSKQLMTPDQSHSF 182
+PL +F+ +++P+ + F
Sbjct: 460 FPLGCASFTDATIVSPNLAPFF 395
>BI418488
Length = 475
Score = 28.1 bits (61), Expect = 2.2
Identities = 21/58 (36%), Positives = 24/58 (41%), Gaps = 20/58 (34%)
Frame = -1
Query: 220 FRQVSQTVDTVLHNP-------------------PRH-LESSSPRRVNHREPVRHGHL 257
FRQ+ V VLH+P P H L S PR +HR PV H HL
Sbjct: 304 FRQMFTLVSPVLHDPSPVRRSMTHNIDHPSGGGIPIHKLISDPPRGRDHRTPVGHRHL 131
>AV775734
Length = 427
Score = 28.1 bits (61), Expect = 2.2
Identities = 13/42 (30%), Positives = 21/42 (49%)
Frame = +3
Query: 214 VSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVRHG 255
+ P H+F + D HN H ++S PR+ + +P R G
Sbjct: 237 LGPMHAFTRQHSQSDIHAHNHYNHNKASPPRQYHE*QPKRLG 362
>TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-like
protein, partial (97%)
Length = 2230
Score = 27.3 bits (59), Expect = 3.7
Identities = 21/60 (35%), Positives = 30/60 (50%)
Frame = +2
Query: 207 LSRGAQSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQST 266
L RG V P H+ R+ ++ P RH ESS R HR P RH H ++ + + +T
Sbjct: 635 LRRGG--VHPCHAPRRAGRS-------PLRHGESSFLRSQQHRRP-RHPHGSLQICKPAT 784
>TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana;}, partial
(40%)
Length = 794
Score = 27.3 bits (59), Expect = 3.7
Identities = 30/118 (25%), Positives = 43/118 (36%), Gaps = 13/118 (11%)
Frame = +2
Query: 152 ITSTSFFDFFYYPLDAFSSKQLMTPDQSHSFGGVTLVTKKEIKRKNSYDSTPTLILSRGA 211
+T T+ F+ F L + T + + F L E+ K SY + + A
Sbjct: 29 LTHTNSFNLFLLILQTQHNTHTHTQNNTQQFIYSILFELNELIIKTSYGFSTRHKQNHKA 208
Query: 212 QSV------SPQHSFRQVSQTVDTVLHNP-------PRHLESSSPRRVNHREPVRHGH 256
++ S QH Q H P P HL+ SPRR H P+ H H
Sbjct: 209 GTLQQLPP*SQQHKASQRFLQTPLPRHPPRRTRPRLPLHLQLPSPRR-RHHPPLPHPH 379
>BP028931
Length = 436
Score = 26.9 bits (58), Expect = 4.8
Identities = 17/40 (42%), Positives = 21/40 (52%)
Frame = +1
Query: 93 YVPYKEVLKHQSTGTKKCYCPFRLRARGTKSSTNMFTKQP 132
Y+P +LK ++TGT K P R R SS N KQP
Sbjct: 118 YIPC--LLKFKNTGTNKVQNPLRDRRP*EYSSINNHIKQP 231
>TC19822 similar to UP|EX13_ARATH (Q9M9P0) Alpha-expansin 13 precursor
(At-EXP13) (AtEx13) (Ath-ExpAlpha-1.22), partial (27%)
Length = 532
Score = 26.9 bits (58), Expect = 4.8
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = -2
Query: 229 TVLHNPPRHLESSSPRRVNHREP 251
T LH+P H + PRR NH P
Sbjct: 429 TFLHHPQPHRSPNPPRRNNHPPP 361
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,811,458
Number of Sequences: 28460
Number of extensions: 85415
Number of successful extensions: 545
Number of sequences better than 10.0: 46
Number of HSP's better than 10.0 without gapping: 538
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 542
length of query: 334
length of database: 4,897,600
effective HSP length: 91
effective length of query: 243
effective length of database: 2,307,740
effective search space: 560780820
effective search space used: 560780820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 55 (25.8 bits)
Lotus: description of TM0022.14