BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0309.4
(116 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC229888 weakly similar to UP|RPCZ_HUMAN (Q9Y2Y1) DNA-directed R... 47 1e-06
TC217015 weakly similar to UP|TFS2_YEAST (P07273) Transcription ... 40 1e-04
TC217014 weakly similar to UP|TFS2_YEAST (P07273) Transcription ... 40 2e-04
TC217677 similar to UP|Q9M9F8 (Q9M9F8) F3F9.7, partial (42%) 32 0.057
TC207495 weakly similar to UP|Q9SMW9 (Q9SMW9) SPL1-Related3 prot... 30 0.22
TC234205 28 0.48
AW348385 similar to GP|21665866|em GDA2 protein {Pisum sativum},... 28 0.63
CF805725 27 1.1
TC233795 27 1.1
TC227116 similar to UP|WAS2_HUMAN (Q9Y6W5) Wiskott-Aldrich syndr... 27 1.1
TC210628 27 1.4
AW595936 27 1.8
TC205822 similar to GB|AAO63369.1|28950891|BT005305 At4g11960 {A... 26 2.4
TC227804 26 3.1
BE331114 similar to GP|9828627|gb| F1N21.13 {Arabidopsis thalian... 25 4.1
BI893087 weakly similar to GP|9759106|dbj| contains similarity t... 25 4.1
TC227652 similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partia... 25 4.1
TC227651 similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partia... 25 4.1
AW394607 25 5.3
BI970836 25 6.9
>TC229888 weakly similar to UP|RPCZ_HUMAN (Q9Y2Y1) DNA-directed RNA
polymerases III 12.5 kDa polypeptide (RNA polymerase
III C11 subunit) (HsC11p) (hRPC11) (My010 protein) ,
partial (41%)
Length = 838
Score = 47.4 bits (111), Expect = 1e-06
Identities = 36/115 (31%), Positives = 49/115 (42%), Gaps = 10/115 (8%)
Frame = +2
Query: 11 FCHYCGTMLTV-------PSVNYAE-CPLCKNRSNIKDIEGKEISYTISAEEIRRELGIE 62
FC CG ML PS + CP + N +I+ K+ +R+E+
Sbjct: 257 FCPSCGNMLQYELPYMGRPSRFFCSACPYVCHIENRVEIKRKQ-------RLVRKEIEPI 415
Query: 63 TIEEQKVQLSKVNKTCEKCGNGEATFYTRQMRSADEGQTTFYACTR--CGHQFQE 115
E+ TC CG+G+A F Q RSADE T FY C C Q++E
Sbjct: 416 FSEDDMTNAPSTEATCPFCGHGKAAFKEFQTRSADEPATLFYKCLNNDCKKQWRE 580
>TC217015 weakly similar to UP|TFS2_YEAST (P07273) Transcription elongation
factor S-II (DNA strand transfer protein alpha)
(STP-alpha) (DNA strand transferase 1) (Pyrimidine
pathway regulatory protein 2), partial (14%)
Length = 673
Score = 40.4 bits (93), Expect = 1e-04
Identities = 15/37 (40%), Positives = 23/37 (61%)
Frame = +1
Query: 78 CEKCGNGEATFYTRQMRSADEGQTTFYACTRCGHQFQ 114
C +CG + T+Y Q RSADE TT+ C C ++++
Sbjct: 265 CGRCGQRKTTYYQMQTRSADEPMTTYVTCVVCNNRWK 375
>TC217014 weakly similar to UP|TFS2_YEAST (P07273) Transcription elongation
factor S-II (DNA strand transfer protein alpha)
(STP-alpha) (DNA strand transferase 1) (Pyrimidine
pathway regulatory protein 2), partial (14%)
Length = 944
Score = 40.0 bits (92), Expect = 2e-04
Identities = 15/37 (40%), Positives = 23/37 (61%)
Frame = +1
Query: 78 CEKCGNGEATFYTRQMRSADEGQTTFYACTRCGHQFQ 114
C +CG + T+Y Q RSADE TT+ C C ++++
Sbjct: 532 CGRCGQRKTTYYQMQTRSADEPMTTYVTCCVCNNRWK 642
>TC217677 similar to UP|Q9M9F8 (Q9M9F8) F3F9.7, partial (42%)
Length = 664
Score = 31.6 bits (70), Expect = 0.057
Identities = 21/81 (25%), Positives = 35/81 (42%), Gaps = 1/81 (1%)
Frame = +1
Query: 12 CHYCGTMLTVP-SVNYAECPLCKNRSNIKDIEGKEISYTISAEEIRRELGIETIEEQKVQ 70
C C + VP S +CP CK + + G + + E G+E IEEQ+V
Sbjct: 217 CTECFLQMKVPNSTRPTQCPFCKTANYAVEYRGVKS---------KEEKGLEQIEEQRVI 369
Query: 71 LSKVNKTCEKCGNGEATFYTR 91
+K+ ++ + E + R
Sbjct: 370 EAKIRMRQQELQDEEERMHKR 432
>TC207495 weakly similar to UP|Q9SMW9 (Q9SMW9) SPL1-Related3 protein
(Fragment), partial (7%)
Length = 1413
Score = 29.6 bits (65), Expect = 0.22
Identities = 15/54 (27%), Positives = 27/54 (49%)
Frame = +2
Query: 38 IKDIEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGEATFYTR 91
+ D + EIS TI+ ++ L +E ++Q + + +C KC N E + R
Sbjct: 92 LADRQRGEISVTIANAIEQQSLRVELKQKQSYLVKRGQSSCAKCANAEIRYNRR 253
>TC234205
Length = 597
Score = 28.5 bits (62), Expect = 0.48
Identities = 16/62 (25%), Positives = 26/62 (41%), Gaps = 3/62 (4%)
Frame = +3
Query: 53 EEIRRELGIETIEEQKVQLSKVNKTCEKCGNGE---ATFYTRQMRSADEGQTTFYACTRC 109
E++ G +T + K +S+ C + G + + S D F +CTRC
Sbjct: 282 EQLSCPTGRDTSRKLKTPISRAPSDCTRYGQNSI**GMLLSPLILSLD*THNLFSSCTRC 461
Query: 110 GH 111
GH
Sbjct: 462 GH 467
>AW348385 similar to GP|21665866|em GDA2 protein {Pisum sativum}, partial
(49%)
Length = 564
Score = 28.1 bits (61), Expect = 0.63
Identities = 14/39 (35%), Positives = 21/39 (52%)
Frame = +2
Query: 7 RDFLFCHYCGTMLTVPSVNYAECPLCKNRSNIKDIEGKE 45
+ +LFC T + ++ YA P C +RSNI I G +
Sbjct: 203 KTYLFC----TFILCLNLAYAAEPTCSHRSNISXISGTD 307
>CF805725
Length = 571
Score = 27.3 bits (59), Expect = 1.1
Identities = 12/41 (29%), Positives = 23/41 (55%)
Frame = -2
Query: 46 ISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGEA 86
+ +A+E+ LG E +EE++V+++ + G GEA
Sbjct: 270 VELVAAADEVETRLGDEVVEERQVEVAGNGEDVPDTGLGEA 148
>TC233795
Length = 713
Score = 27.3 bits (59), Expect = 1.1
Identities = 12/29 (41%), Positives = 14/29 (47%)
Frame = +1
Query: 8 DFLFCHYCGTMLTVPSVNYAECPLCKNRS 36
D +CHY + T P Y CP C N S
Sbjct: 460 DDYWCHYILMLSTEPLNGYLVCPFCFNFS 546
>TC227116 similar to UP|WAS2_HUMAN (Q9Y6W5) Wiskott-Aldrich syndrome protein
family member 2 (WASP-family protein member 2)
(Verprolin homology domain-containing protein 2),
partial (5%)
Length = 1154
Score = 27.3 bits (59), Expect = 1.1
Identities = 21/76 (27%), Positives = 31/76 (40%)
Frame = +2
Query: 26 YAECPLCKNRSNIKDIEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGNGE 85
+A CPLC+ R +D EG + T EI L + T E+ + T +
Sbjct: 446 HASCPLCRFRLEEEDEEGGDGVMTKIRREIIARLTVLTEED----FYGLRTTLNHIASRH 613
Query: 86 ATFYTRQMRSADEGQT 101
A + R A G+T
Sbjct: 614 ALIQENENRGAQIGET 661
>TC210628
Length = 633
Score = 26.9 bits (58), Expect = 1.4
Identities = 15/43 (34%), Positives = 19/43 (43%), Gaps = 3/43 (6%)
Frame = -1
Query: 9 FLFCHYCG---TMLTVPSVNYAECPLCKNRSNIKDIEGKEISY 48
F+ C C T L VP + A PLC IKD+ S+
Sbjct: 255 FMHCRSCSSSFTKLMVPPIIEA*SPLCT*EGGIKDLSTARYSF 127
>AW595936
Length = 400
Score = 26.6 bits (57), Expect = 1.8
Identities = 10/17 (58%), Positives = 13/17 (75%)
Frame = -2
Query: 33 KNRSNIKDIEGKEISYT 49
KN N+KD+EGKE+ T
Sbjct: 393 KNSDNVKDLEGKELKGT 343
>TC205822 similar to GB|AAO63369.1|28950891|BT005305 At4g11960 {Arabidopsis
thaliana;} , partial (79%)
Length = 1373
Score = 26.2 bits (56), Expect = 2.4
Identities = 9/33 (27%), Positives = 15/33 (45%)
Frame = +1
Query: 78 CEKCGNGEATFYTRQMRSADEGQTTFYACTRCG 110
C CG +F+ + ++ G T C+ CG
Sbjct: 925 CPNCGTENTSFFGTILSISNGGSTNTVKCSNCG 1023
>TC227804
Length = 1344
Score = 25.8 bits (55), Expect = 3.1
Identities = 15/43 (34%), Positives = 23/43 (52%)
Frame = +1
Query: 41 IEGKEISYTISAEEIRRELGIETIEEQKVQLSKVNKTCEKCGN 83
++GKE Y I EEI + IE KV+L+ + K + G+
Sbjct: 244 LDGKEGMYEIHNEEILEKDVEHAIELSKVRLANIVKRPNRSGS 372
>BE331114 similar to GP|9828627|gb| F1N21.13 {Arabidopsis thaliana}, partial
(6%)
Length = 322
Score = 25.4 bits (54), Expect = 4.1
Identities = 11/34 (32%), Positives = 16/34 (46%)
Frame = -3
Query: 76 KTCEKCGNGEATFYTRQMRSADEGQTTFYACTRC 109
K+C + G+G AT + R G T +C C
Sbjct: 161 KSCVQSGSGFATNFLRSCYKYSSGSCTHTSCFSC 60
>BI893087 weakly similar to GP|9759106|dbj| contains similarity to C3HC4-type
RING zinc finger protein~gene_id:MJJ3.23 {Arabidopsis
thaliana}, partial (7%)
Length = 260
Score = 25.4 bits (54), Expect = 4.1
Identities = 10/32 (31%), Positives = 21/32 (65%)
Frame = -1
Query: 25 NYAECPLCKNRSNIKDIEGKEISYTISAEEIR 56
+++ CPLC+ R DI K +++++S+ +R
Sbjct: 221 SHSTCPLCRRRVEAGDI--KNLNFSLSSRFLR 132
>TC227652 similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partial (35%)
Length = 1209
Score = 25.4 bits (54), Expect = 4.1
Identities = 8/22 (36%), Positives = 11/22 (49%)
Frame = +3
Query: 11 FCHYCGTMLTVPSVNYAECPLC 32
FCH C +T + CP+C
Sbjct: 96 FCHVCSQRITCSDESEPFCPIC 161
>TC227651 similar to UP|Q8LPN7 (Q8LPN7) AT3g19950/MPN9_19, partial (33%)
Length = 1364
Score = 25.4 bits (54), Expect = 4.1
Identities = 8/22 (36%), Positives = 11/22 (49%)
Frame = +1
Query: 11 FCHYCGTMLTVPSVNYAECPLC 32
FCH C +T + CP+C
Sbjct: 97 FCHVCSQRITCSDESEPFCPMC 162
>AW394607
Length = 415
Score = 25.0 bits (53), Expect = 5.3
Identities = 10/25 (40%), Positives = 12/25 (48%)
Frame = +1
Query: 4 SRERDFLFCHYCGTMLTVPSVNYAE 28
S E CH+CGT LT Y +
Sbjct: 307 SLEMGLCHCHHCGTKLTTKKSPYMQ 381
>BI970836
Length = 564
Score = 24.6 bits (52), Expect = 6.9
Identities = 11/26 (42%), Positives = 13/26 (49%)
Frame = -1
Query: 22 PSVNYAECPLCKNRSNIKDIEGKEIS 47
P V CP N+ IKD+ EIS
Sbjct: 132 PEVTLKNCPWMYNQMQIKDLPPPEIS 55
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.133 0.400
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,713,605
Number of Sequences: 63676
Number of extensions: 62659
Number of successful extensions: 424
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 422
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 423
length of query: 116
length of database: 12,639,632
effective HSP length: 92
effective length of query: 24
effective length of database: 6,781,440
effective search space: 162754560
effective search space used: 162754560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Lotus: description of TM0309.4