TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= TM0282.7
         (463 letters)

Database: GMGI 
           63,676 sequences; 37,918,896 total letters

Searching..................................................done

                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC225082 similar to UP|Q9M6U1 (Q9M6U1) Homogentisate 1,2-dioxyge...   565  e-161
TC225076 similar to UP|Q9M6U1 (Q9M6U1) Homogentisate 1,2-dioxyge...   287  e-128
BI320272 similar to GP|8131905|gb| homogentisate 1 2-dioxygenase...   241  6e-64
BU926487 homologue to GP|8131905|gb|A homogentisate 1 2-dioxygen...   204  9e-53
AI431154                                                               64  2e-10
TC210913 similar to UP|O13524 (O13524) B2-aldehyde-forming enzym...    28  6.6
TC211755 similar to UP|Q9SAK1 (Q9SAK1) T8K14.10, partial (22%)         28  8.6

>TC225082 similar to UP|Q9M6U1 (Q9M6U1) Homogentisate 1,2-dioxygenase
           (Fragment) , partial (56%)
          Length = 1234

 Score =  565 bits (1455), Expect = e-161
 Identities = 262/284 (92%), Positives = 272/284 (95%)
 Frame = +3

Query: 168 LFITTECGRLKVSPGEIAMLPQGFRFTVDLPDGPSRGYVAEIFGTHFQLPDLGPIGANGL 227
           L +TTECGRLKVSPGEIA+LPQGFRF+V+LPDGPSRGYVAEIFGTHFQLPDLGPIGANGL
Sbjct: 21  LLVTTECGRLKVSPGEIAILPQGFRFSVNLPDGPSRGYVAEIFGTHFQLPDLGPIGANGL 200

Query: 228 AAPRDFLVPTAWFEDKSYPGYTIVQKFGGELFSAVQDFSPFNVVAWRGNYVPYKYDLSKF 287
           A+PRDFLVPTAWFEDKSYPGYTIVQKFGGELF AVQDFSPFNVVAW GNYVPYKYDL+KF
Sbjct: 201 ASPRDFLVPTAWFEDKSYPGYTIVQKFGGELFDAVQDFSPFNVVAWHGNYVPYKYDLNKF 380

Query: 288 CPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEF 347
           CPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEF
Sbjct: 381 CPYNTVLFDHSDPSINTVLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEF 560

Query: 348 MGLIHGGYEAKVDGFLPGGASLHSCMTPHGPDTKSYEATIARGNDVGPNKITDTMAFMFE 407
           MGLIHGGYEAK DGFLPGGASLHSCMTPHGPDTKSYEATIARGNDVGP KITDTMAFMFE
Sbjct: 561 MGLIHGGYEAKADGFLPGGASLHSCMTPHGPDTKSYEATIARGNDVGPCKITDTMAFMFE 740

Query: 408 SCLIPRISRWALESPFLDHDYYQCWIGLRSHFNVTEISPRTPTL 451
           S LIPRIS+WA ESPFLD DYYQCWIGL+SHF VT+ SP  P+L
Sbjct: 741 SSLIPRISQWASESPFLDQDYYQCWIGLKSHFAVTKTSPENPSL 872


>TC225076 similar to UP|Q9M6U1 (Q9M6U1) Homogentisate 1,2-dioxygenase
           (Fragment) , partial (48%)
          Length = 1391

 Score =  287 bits (735), Expect(2) = e-128
 Identities = 133/147 (90%), Positives = 136/147 (92%)
 Frame = +1

Query: 305 VLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIHGGYEAKVDGFLP 364
           VLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIHGGYEAK DGFLP
Sbjct: 289 VLTAPTDKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIHGGYEAKADGFLP 468

Query: 365 GGASLHSCMTPHGPDTKSYEATIARGNDVGPNKITDTMAFMFESCLIPRISRWALESPFL 424
           GGASLH+CMTPHGPDTKSYEATIARGND GP KITDTMAFMFES LIPRIS+WALESPFL
Sbjct: 469 GGASLHNCMTPHGPDTKSYEATIARGNDGGPCKITDTMAFMFESSLIPRISQWALESPFL 648

Query: 425 DHDYYQCWIGLRSHFNVTEISPRTPTL 451
           D DYYQCWIGL+SHF VTE SP    L
Sbjct: 649 DQDYYQCWIGLKSHFTVTETSPENTNL 729



 Score =  190 bits (482), Expect(2) = e-128
 Identities = 86/96 (89%), Positives = 92/96 (95%)
 Frame = +2

Query: 186 MLPQGFRFTVDLPDGPSRGYVAEIFGTHFQLPDLGPIGANGLAAPRDFLVPTAWFEDKSY 245
           ++P GFRF+V+L DGPSRGYVAEIFGTHFQLPDLGPIGANGLA+PRDFLVP+AWFEDKSY
Sbjct: 2   IIPHGFRFSVNLSDGPSRGYVAEIFGTHFQLPDLGPIGANGLASPRDFLVPSAWFEDKSY 181

Query: 246 PGYTIVQKFGGELFSAVQDFSPFNVVAWRGNYVPYK 281
           PGYTIVQKFGGELF AVQDFSPFNVVAW GNYVPYK
Sbjct: 182 PGYTIVQKFGGELFDAVQDFSPFNVVAWHGNYVPYK 289


>BI320272 similar to GP|8131905|gb| homogentisate 1 2-dioxygenase
           {Lycopersicon esculentum}, partial (24%)
          Length = 389

 Score =  241 bits (614), Expect = 6e-64
 Identities = 113/129 (87%), Positives = 120/129 (92%)
 Frame = +2

Query: 2   EHSTAGDDFNYLSGFGNHFSSEALAGALPEAQNSPLICPYGLYAEQISGTSFTSPRNLNL 61
           E+   G +F YLSGFGNHFSSEALAGALP AQNSPL+CPYGLYAEQISGTSFTSPRN NL
Sbjct: 2   ENPIDGGEFVYLSGFGNHFSSEALAGALPVAQNSPLVCPYGLYAEQISGTSFTSPRNRNL 181

Query: 62  FSWFYRVKPSVTHEPFKPRVPCNGRILSEFNHSNSSTNPTQLRWKPMDSPDSPTDFIDGL 121
           FSWFYR+KPSVTHEPFKPRVP NGRILSEFN+SNSS NPTQLRWKP+D+PDSPTDFIDGL
Sbjct: 182 FSWFYRIKPSVTHEPFKPRVPGNGRILSEFNNSNSSANPTQLRWKPLDAPDSPTDFIDGL 361

Query: 122 STVCGSGSS 130
           STVCGSGSS
Sbjct: 362 STVCGSGSS 388


>BU926487 homologue to GP|8131905|gb|A homogentisate 1 2-dioxygenase
           {Lycopersicon esculentum}, partial (20%)
          Length = 445

 Score =  204 bits (518), Expect = 9e-53
 Identities = 93/96 (96%), Positives = 93/96 (96%)
 Frame = +3

Query: 261 AVQDFSPFNVVAWRGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDF 320
           AVQDFSPFNVVAW GNYVP KYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDF
Sbjct: 117 AVQDFSPFNVVAWHGNYVP*KYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALLDF 296

Query: 321 VIFPPRWLVAEHTFRPPYYHRNCMSEFMGLIHGGYE 356
           VIFPPRWLVAEHTF PPYYHRNCMSEFMGLIHGGYE
Sbjct: 297 VIFPPRWLVAEHTFLPPYYHRNCMSEFMGLIHGGYE 404



 Score = 30.4 bits (67), Expect = 1.7
 Identities = 11/14 (78%), Positives = 13/14 (92%)
 Frame = +2

Query: 141 YTANKSMNNCAFCN 154
           Y ANKSM+NCAFC+
Sbjct: 80  YNANKSMDNCAFCS 121


>AI431154 
          Length = 130

 Score = 63.5 bits (153), Expect = 2e-10
 Identities = 28/43 (65%), Positives = 31/43 (71%)
 Frame = +2

Query: 336 PPYYHRNCMSEFMGLIHGGYEAKVDGFLPGGASLHSCMTPHGP 378
           PPY +R    EFMG I GGY AK DGFLP GA LHSC+T +GP
Sbjct: 2   PPYDYRKWAKEFMGPIQGGY*AKADGFLPAGARLHSCLTTYGP 130


>TC210913 similar to UP|O13524 (O13524) B2-aldehyde-forming enzyme, partial
           (10%)
          Length = 593

 Score = 28.5 bits (62), Expect = 6.6
 Identities = 17/45 (37%), Positives = 23/45 (50%)
 Frame = +1

Query: 274 RGNYVPYKYDLSKFCPYNTVLFDHSDPSINTVLTAPTDKPGVALL 318
           RG YV Y +D     P N V  +H     NT++ A  D PGV ++
Sbjct: 394 RGGYVTYVHDSCM*VPGNAV--NHLY*LQNTIIFAVIDPPGVVIM 522


>TC211755 similar to UP|Q9SAK1 (Q9SAK1) T8K14.10, partial (22%)
          Length = 1127

 Score = 28.1 bits (61), Expect = 8.6
 Identities = 15/48 (31%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
 Frame = +1

Query: 300 PSINTVLTAPT-DKPGVALLDFVIFPPRWLVAEHTFRPPYYHRNCMSE 346
           PS++   +AP+ + P +  L  V+FPP    + H  +PP Y   C+++
Sbjct: 562 PSLSPPKSAPSLNPPPLVYLPPVVFPPPPSPSTHRKKPPQYALWCVAK 705


  Database: GMGI
    Posted date:  Oct 22, 2004  4:58 PM
  Number of letters in database: 37,918,896
  Number of sequences in database:  63,676
  
Lambda     K      H
   0.321    0.139    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,194,146
Number of Sequences: 63676
Number of extensions: 390720
Number of successful extensions: 1708
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 1681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1707
length of query: 463
length of database: 12,639,632
effective HSP length: 101
effective length of query: 362
effective length of database: 6,208,356
effective search space: 2247424872
effective search space used: 2247424872
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 61 (28.1 bits)