BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0083.3
(287 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CF922212 52 3e-07
CA783280 weakly similar to PIR|T01871|T01 RNA-directed DNA polym... 49 3e-06
BI702277 49 3e-06
BQ630369 42 5e-06
BM891241 45 4e-05
TC233846 31 5e-04
BU090318 weakly similar to GP|9743345|gb| F21J9.30 {Arabidopsis ... 37 0.013
TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {A... 34 0.067
TC221865 34 0.087
BM891637 32 0.33
AI965344 similar to GP|13277342|emb Germin-like protein {Pisum s... 32 0.43
BM309783 31 0.74
BQ740812 30 0.97
TC230187 similar to UP|O23280 (O23280) RING-H2 finger protein RH... 29 2.8
TC228580 weakly similar to UP|Q944E5 (Q944E5) Cellulose synthase... 28 3.7
TC224847 weakly similar to UP|Q96538 (Q96538) Acyl-CoA synthetas... 28 4.8
TC224933 UP|Q6K853 (Q6K853) 40S ribosomal protein S30-like, comp... 28 4.8
TC225984 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer pro... 28 6.3
TC215814 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precu... 28 6.3
BG239687 28 6.3
>CF922212
Length = 445
Score = 52.0 bits (123), Expect = 3e-07
Identities = 32/106 (30%), Positives = 48/106 (45%), Gaps = 7/106 (6%)
Frame = -2
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAW-------KLGSRIFITKYLKGSSIFSY 125
VNW + LPK + GLG R R+ N +LLGK W +LG+R+ +KY + ++
Sbjct: 354 VNWDSVCLPKEKEGLGNRDLRKFNYALLGKRRWNLFHHQGELGARVLDSKYKRWRNLDEE 175
Query: 126 ELTGSSSYVWKGLVKAFSVLRDGLSFAIHDGCTSFWYSDWTGLGKL 171
S S+ W+ +SF H W+ T G+L
Sbjct: 174 RRVKSESFWWQ-----------EISFITHSTEDGSWFEKRTKTGRL 70
>CA783280 weakly similar to PIR|T01871|T01 RNA-directed DNA polymerase
homolog T24M8.8 - Arabidopsis thaliana, partial (4%)
Length = 456
Score = 48.9 bits (115), Expect = 3e-06
Identities = 27/82 (32%), Positives = 41/82 (49%), Gaps = 7/82 (8%)
Frame = +2
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKL-------GSRIFITKYLKGSSIFSY 125
VNWK + LPK +GGLG++ R N +LLGK W L +++ +KY ++
Sbjct: 104 VNWKTVCLPKAKGGLGIKDLRTFNTTLLGKWRWDLFYIQQEPWAKVLQSKYGGWRALEEG 283
Query: 126 ELTGSSSYVWKGLVKAFSVLRD 147
S WK L+K + R+
Sbjct: 284 SSGSKDSAWWKDLIKTQQLQRN 349
>BI702277
Length = 420
Score = 48.5 bits (114), Expect = 3e-06
Identities = 21/63 (33%), Positives = 37/63 (58%)
Frame = +2
Query: 3 GEILATTTSVMGPVLTPVLSEALCLRWAIGMARDLGFRHVWFDTDCLVLFDYWRRSDEGR 62
GE+LA+ T + +P ++E L +W+I MA L ++V+F+TDC + WR + +
Sbjct: 2 GEVLASATRLEDQCYSPQIAEYLAFKWSIQMANQLLLQNVFFETDCREIASAWRNASQRI 181
Query: 63 SHL 65
H+
Sbjct: 182VHI 190
>BQ630369
Length = 389
Score = 42.0 bits (97), Expect(2) = 5e-06
Identities = 21/49 (42%), Positives = 27/49 (54%)
Frame = +1
Query: 159 SFWYSDWTGLGKLCALVPFVHISDTALTISDVWVEGSWNLSMLYTELPD 207
SFW+S+W G +C FVHI LTIS+V NL+ L LP+
Sbjct: 223 SFWFSNWLGSWPICLDENFVHIEHAHLTISEVRENVQRNLAKLRAPLPE 369
Score = 25.4 bits (54), Expect(2) = 5e-06
Identities = 10/18 (55%), Positives = 13/18 (71%)
Frame = +3
Query: 131 SSYVWKGLVKAFSVLRDG 148
+SYVW +VK+ LRDG
Sbjct: 135 ASYVWCSIVKSVDKLRDG 188
>BM891241
Length = 407
Score = 45.1 bits (105), Expect = 4e-05
Identities = 25/60 (41%), Positives = 34/60 (56%), Gaps = 7/60 (11%)
Frame = -2
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKLGS-------RIFITKYLKGSSIFSY 125
V W+ + LPK +GGLG++ + N++LLGK W L S RI +KY G S F Y
Sbjct: 373 VKWEVVCLPKTKGGLGIKDISKFNIALLGKWIWALASDQQQLWARIINSKY-GGWSDFQY 197
>TC233846
Length = 424
Score = 31.2 bits (69), Expect(2) = 5e-04
Identities = 17/47 (36%), Positives = 29/47 (61%), Gaps = 2/47 (4%)
Frame = -2
Query: 162 YSDWTGLGKLCALV--PFVHISDTALTISDVWVEGSWNLSMLYTELP 206
++ W+GL + A + P+V+ISD L + D+ +G LS ++T LP
Sbjct: 279 WASWSGL*LVVAALFGPYVNISDVDLCLKDLIHDGE*MLSDIHTTLP 139
Score = 29.3 bits (64), Expect(2) = 5e-04
Identities = 11/23 (47%), Positives = 15/23 (64%), Gaps = 1/23 (4%)
Frame = -3
Query: 224 VDDCWVW-NSNPAGYSCSDAYQW 245
++D WVW NSN YS + Y+W
Sbjct: 86 INDSWVWSNSNNGIYSAASGYRW 18
>BU090318 weakly similar to GP|9743345|gb| F21J9.30 {Arabidopsis thaliana},
partial (1%)
Length = 422
Score = 36.6 bits (83), Expect = 0.013
Identities = 28/102 (27%), Positives = 45/102 (43%), Gaps = 10/102 (9%)
Frame = +3
Query: 74 NWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKLGS-------RIFITKYLKGSSIFS-Y 125
+WK + PK GGLG+R N SLL K + + R+ +KY G I
Sbjct: 18 SWKALCSPKKSGGLGLRLLSHMNKSLLMKST*NMCTKPNDL*VRVVRSKYK*GKDILP*I 197
Query: 126 ELTGSSSYVWKGLVKAF-SVLRDGLSFAIHDG-CTSFWYSDW 165
+ + + W+G+V + + D ++ I +G FW W
Sbjct: 198 DPKKTGTNFWQGIVHCWEKITHDNIARKIGNGRSVHFWKDVW 323
>TC216314 similar to GB|AAP68269.1|31711826|BT008830 At1g70320 {Arabidopsis
thaliana;} , partial (18%)
Length = 759
Score = 34.3 bits (77), Expect = 0.067
Identities = 15/37 (40%), Positives = 19/37 (50%)
Frame = +2
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAWKLGS 109
V W I PK GGLG++ N +L G+ W L S
Sbjct: 161 VKWADICNPKIDGGLGIKDLSNFNAALRGRWIWGLAS 271
>TC221865
Length = 1045
Score = 33.9 bits (76), Expect = 0.087
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = -3
Query: 219 PSHETVDDCWVWNSNPAG-YSCSDAYQWLR 247
P H + DCWVW S P G YS AY ++
Sbjct: 689 PIHRHLTDCWVWKSEPNGHYSTRSAYHLMQ 600
>BM891637
Length = 427
Score = 32.0 bits (71), Expect = 0.33
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 7/51 (13%)
Frame = -1
Query: 73 VNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAW-------KLGSRIFITKY 116
++W+Q + GGLG++ + N +LL K W +L +RI I+KY
Sbjct: 403 ISWRQCCASGDVGGLGIKDIKILNNALLIKWKWLMFHQPHQLWNRILISKY 251
>AI965344 similar to GP|13277342|emb Germin-like protein {Pisum sativum},
partial (45%)
Length = 355
Score = 31.6 bits (70), Expect = 0.43
Identities = 22/62 (35%), Positives = 31/62 (49%), Gaps = 4/62 (6%)
Frame = +1
Query: 208 PYKPTITSMQGPSHETVDDCWVWN----SNPAGYSCSDAYQWLRDWTVFRVLSVLGSMSS 263
P+ TI S+ SH +V D V + + PAGYSC + D V+ L G+ S+
Sbjct: 49 PFFNTIVSLLYLSHASVVDACVADYTGPNGPAGYSCKSPAKVTVDDFVYSGLGTAGNTSN 228
Query: 264 II 265
II
Sbjct: 229 II 234
>BM309783
Length = 433
Score = 30.8 bits (68), Expect = 0.74
Identities = 15/48 (31%), Positives = 24/48 (49%)
Frame = +2
Query: 58 SDEGRSHLGTVVRDCVNWKQIVLPKNQGGLGVRSTRESNVSLLGKLAW 105
+D GR H V WK +++P + GG+G+ N + L K+ W
Sbjct: 14 NDGGRKH------HVVGWKAMIMP*HSGGVGMCDLTIMNQACLMKIGW 139
>BQ740812
Length = 421
Score = 30.4 bits (67), Expect = 0.97
Identities = 11/24 (45%), Positives = 14/24 (57%), Gaps = 1/24 (4%)
Frame = -2
Query: 227 CWVWNSNPAGY-SCSDAYQWLRDW 249
CW+WNS P+G+ YQ L W
Sbjct: 168 CWIWNSEPSGWLEFPQEYQQLERW 97
>TC230187 similar to UP|O23280 (O23280) RING-H2 finger protein RHF1A, partial
(17%)
Length = 789
Score = 28.9 bits (63), Expect = 2.8
Identities = 12/32 (37%), Positives = 21/32 (65%)
Frame = +3
Query: 2 NGEILATTTSVMGPVLTPVLSEALCLRWAIGM 33
NG++L + S+ GP+ T +L +L R A+G+
Sbjct: 291 NGQVLLSLLSIAGPLQTSLLQGSLLKRMALGV 386
>TC228580 weakly similar to UP|Q944E5 (Q944E5) Cellulose synthase-like
protein OsCslA9, partial (14%)
Length = 394
Score = 28.5 bits (62), Expect = 3.7
Identities = 21/79 (26%), Positives = 35/79 (43%), Gaps = 4/79 (5%)
Frame = +1
Query: 181 SDTALTISDVWVEGSWNLSMLYTELPDPYKPT----ITSMQGPSHETVDDCWVWNSNPAG 236
S + ++S++W SW+L ML P P K + S PS + V C+ +S +
Sbjct: 142 SSSCSSLSNLWPSSSWHLDMLAPLFPTPKKDSALHWAISASVPSCQRVL*CFQHSSGKSQ 321
Query: 237 YSCSDAYQWLRDWTVFRVL 255
+ W+ F+VL
Sbjct: 322 LVTIKK----KGWSFFKVL 366
>TC224847 weakly similar to UP|Q96538 (Q96538) Acyl-CoA synthetase , partial
(17%)
Length = 704
Score = 28.1 bits (61), Expect = 4.8
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -1
Query: 95 SNVSLLGKLAWKLGSRIFIT 114
SN+ +LGK W+LG+R F T
Sbjct: 644 SNIIVLGKYTWQLGTRQFCT 585
>TC224933 UP|Q6K853 (Q6K853) 40S ribosomal protein S30-like, complete
Length = 935
Score = 28.1 bits (61), Expect = 4.8
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -1
Query: 95 SNVSLLGKLAWKLGSRIFIT 114
SN+ +LGK W+LG+R F T
Sbjct: 884 SNIIVLGKYTWQLGTRQFCT 825
>TC225984 weakly similar to UP|Q6Z387 (Q6Z387) Lipid transfer protein-like,
partial (28%)
Length = 1140
Score = 27.7 bits (60), Expect = 6.3
Identities = 13/41 (31%), Positives = 21/41 (50%)
Frame = +2
Query: 147 DGLSFAIHDGCTSFWYSDWTGLGKLCALVPFVHISDTALTI 187
+G S + ++ +S W W LC ++PFV IS L +
Sbjct: 593 NGTSTSANNNSSSGWGKRWLVTEVLCGILPFVFISHLFLFV 715
>TC215814 similar to UP|Q9AR81 (Q9AR81) Germin-like protein precursor,
partial (95%)
Length = 1087
Score = 27.7 bits (60), Expect = 6.3
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Frame = +1
Query: 213 ITSMQGPSHETVDDCWVWN----SNPAGYSCSDAYQWLRDWTVFRVLSVLGSMSSII 265
I S+ SH +V D V + + PAGYSC + D V+ L G+ S+II
Sbjct: 139 ILSLLSLSHASVVDFCVADYTGPNGPAGYSCKSPAKVTVDDFVYSGLGTAGNTSNII 309
>BG239687
Length = 443
Score = 27.7 bits (60), Expect = 6.3
Identities = 11/20 (55%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
Frame = -3
Query: 225 DDCWVWNSNPA-GYSCSDAY 243
+D WVW SN + GY+ DAY
Sbjct: 231 EDSWVWKSNESGGYNVRDAY 172
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.321 0.136 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,436,014
Number of Sequences: 63676
Number of extensions: 286266
Number of successful extensions: 1289
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 1281
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1289
length of query: 287
length of database: 12,639,632
effective HSP length: 96
effective length of query: 191
effective length of database: 6,526,736
effective search space: 1246606576
effective search space used: 1246606576
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 58 (26.9 bits)
Lotus: description of TM0083.3