BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= TM0022.14
(334 letters)
Database: GMGI
63,676 sequences; 37,918,896 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BG839904 87 8e-18
BU545334 64 1e-10
AI736706 49 4e-06
CF920253 37 0.010
TC211617 37 0.013
TC216098 similar to UP|PI27_ARATH (P93004) Aquaporin PIP2.7 (Pla... 34 0.081
TC232658 similar to UP|Q72KT5 (Q72KT5) PmbA protein, partial (4%) 33 0.14
TC220307 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F1... 30 1.2
TC226798 weakly similar to UP|Q8W575 (Q8W575) AT5g14250/F18O22_4... 29 2.6
TC231388 weakly similar to UP|Q9LSB9 (Q9LSB9) Emb|CAA16573.1 (At... 29 3.4
CO984933 28 4.4
TC225016 similar to UP|Q39835 (Q39835) Extensin, partial (32%) 28 4.4
BG839202 similar to GP|19310423|gb At1g10390/F14N23_29 {Arabidop... 28 5.8
TC216994 weakly similar to GB|AAD18096.1|4337175|T31J12 ESTs gb|... 28 5.8
TC226825 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydra... 28 5.8
TC223427 28 7.6
TC203287 weakly similar to UP|Q41645 (Q41645) Extensin (Fragment... 28 7.6
BG790175 similar to PIR|T09854|T098 proline-rich cell wall prote... 28 7.6
TC233438 similar to UP|Q6XD84 (Q6XD84) SR RNA-binding protein, p... 28 7.6
>BG839904
Length = 712
Score = 87.4 bits (215), Expect = 8e-18
Identities = 41/105 (39%), Positives = 65/105 (61%), Gaps = 9/105 (8%)
Frame = -3
Query: 35 DYTQSFTTDKVFASRDEILEWARNLGKQHGFIIVITRSDNGGL--------KRKTFMILG 86
D + +F T +VF SR+ +L WAR++ K++GF+++I RS+ +RKTF+I+G
Sbjct: 518 DCSDAFNTTEVFPSREAMLNWARDVAKENGFVLIILRSETSTTCTRSTRCNQRKTFVIMG 339
Query: 87 CERCDKY-VPYKEVLKHQSTGTKKCYCPFRLRARGTKSSTNMFTK 130
C+R KY PYK L + +GT+KC CPF+L+ + K + K
Sbjct: 338 CDRSGKYRGPYKNELSRKVSGTRKCECPFKLKGKALKKAEGWIVK 204
>BU545334
Length = 556
Score = 63.5 bits (153), Expect = 1e-10
Identities = 32/88 (36%), Positives = 54/88 (61%), Gaps = 2/88 (2%)
Frame = -1
Query: 34 VDYTQSFTTDKVFASRDEILEWARNLGKQHGFIIVITRSD--NGGLKRKTFMILGCERCD 91
VD + +F T +VF +RD +L+ R++ ++GF VI RSD G R +F+++GCER
Sbjct: 457 VDCSYAFNTXQVFGTRDYVLQ*XRSVAHENGFAAVIMRSDTHTGSRGRSSFVLIGCERNG 278
Query: 92 KYVPYKEVLKHQSTGTKKCYCPFRLRAR 119
+Y + ++ G++K CPF+LR +
Sbjct: 277 QYKCRNKXXVIRAIGSRKYGCPFKLRGK 194
>AI736706
Length = 426
Score = 48.5 bits (114), Expect = 4e-06
Identities = 26/88 (29%), Positives = 46/88 (51%)
Frame = +3
Query: 7 SEVDKPEEKPLTGKLVRVEDVQDEPLAVDYTQSFTTDKVFASRDEILEWARNLGKQHGFI 66
SE+ K P+ G + + +VD + +F T +VF SR+ +L WAR++ K++GF+
Sbjct: 186 SEMSKEVTIPIDGAIDK---------SVDCSDAFNTTEVFPSREAMLNWARDVAKENGFV 338
Query: 67 IVITRSDNGGLKRKTFMILGCERCDKYV 94
++I RS+ K C + +V
Sbjct: 339 LIILRSETSTRSTKYTRSTRCNQRKTFV 422
>CF920253
Length = 856
Score = 37.4 bits (85), Expect = 0.010
Identities = 19/51 (37%), Positives = 32/51 (62%), Gaps = 2/51 (3%)
Frame = -2
Query: 45 VFASRDEILEWARNLGKQHGFIIVITRSD-NGGLKRKT-FMILGCERCDKY 93
VF RD++L W + + + F+ +I D N G++ KT F+++GCER +Y
Sbjct: 282 VFVIRDDVL*WTKFVAYEIEFVTLIMSLDTNTGMREKTSFVLIGCERNGQY 130
>TC211617
Length = 534
Score = 37.0 bits (84), Expect = 0.013
Identities = 15/34 (44%), Positives = 21/34 (61%)
Frame = -1
Query: 86 GCERCDKYVPYKEVLKHQSTGTKKCYCPFRLRAR 119
GCER KY K+ + T T+KC CPF++R +
Sbjct: 534 GCERSGKYKCRKKEFVRRDTCTRKCGCPFKIRGK 433
>TC216098 similar to UP|PI27_ARATH (P93004) Aquaporin PIP2.7 (Plasma membrane
intrinsic protein 3) (Salt-stress induced major
intrinsis protein), partial (81%)
Length = 838
Score = 34.3 bits (77), Expect = 0.081
Identities = 29/101 (28%), Positives = 39/101 (37%)
Frame = +1
Query: 179 SHSFGGVTLVTKKEIKRKNSYDSTPTLILSRGAQSVSPQHSFRQVSQTVDTVLHNPPRHL 238
SH T +KR+N S P G + S SFR +L +P R +
Sbjct: 94 SHKTSKQLTPTLTPVKRENVEGSEPRRPAEEGLRRPSSSPSFRPCRDQALVLLQSPHRRV 273
Query: 239 ESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQLQRIIL 279
+P + HR RH H R T RRR R++L
Sbjct: 274 HCLTPLPLRHR---RHHHRPQETDR--TMRRRWPSRHRMVL 381
>TC232658 similar to UP|Q72KT5 (Q72KT5) PmbA protein, partial (4%)
Length = 451
Score = 33.5 bits (75), Expect = 0.14
Identities = 20/50 (40%), Positives = 27/50 (54%)
Frame = +1
Query: 227 VDTVLHNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQLQR 276
+ T LH PPRHL+ SP R N R+ R + V R S RR + + +R
Sbjct: 58 LQTPLHFPPRHLQRFSPHRANPRKQRR-----IRVGRPSQRRRMDRKWRR 192
>TC220307 similar to GB|AAM65362.1|21655301|AY103310 At2g37550/F13M22.5
{Arabidopsis thaliana;} , partial (28%)
Length = 602
Score = 30.4 bits (67), Expect = 1.2
Identities = 19/56 (33%), Positives = 27/56 (47%)
Frame = +1
Query: 231 LHNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQLQRIILTSINHTR 286
LH P ++ PRR + P+RH L + Q RR+ Q QRI T ++ R
Sbjct: 256 LHVPRMLRQAPRPRRPHLLRPIRHHGLLVRHPDQENGSRRQRQAQRIPRTVLHPQR 423
>TC226798 weakly similar to UP|Q8W575 (Q8W575) AT5g14250/F18O22_40, partial
(86%)
Length = 1572
Score = 29.3 bits (64), Expect = 2.6
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +3
Query: 212 QSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVNH 248
+SV P +++VS++ T LH P R +PR H
Sbjct: 24 RSVFPPSRYKRVSESQLTALHGPSRSFGRPNPRAFEH 134
>TC231388 weakly similar to UP|Q9LSB9 (Q9LSB9) Emb|CAA16573.1 (At3g15920),
partial (10%)
Length = 684
Score = 28.9 bits (63), Expect = 3.4
Identities = 17/63 (26%), Positives = 31/63 (48%)
Frame = +1
Query: 25 EDVQDEPLAVDYTQSFTTDKVFASRDEILEWARNLGKQHGFIIVITRSDNGGLKRKTFMI 84
+DV+D +AV+ T+ TTD + DE+ + H F+ DN L+++ +
Sbjct: 238 QDVEDTVVAVEETRDITTDNTGKTHDELRKML-----AHMFV------DNASLRKQINSV 384
Query: 85 LGC 87
+ C
Sbjct: 385 IRC 393
>CO984933
Length = 754
Score = 28.5 bits (62), Expect = 4.4
Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 3/62 (4%)
Frame = -2
Query: 246 VNHREPVRHGHLTMGVVRQSTHRRREGQLQRIILTSINHTRQL---SQYACCMNEMPVDK 302
V+ E + H + V+R S H + E + + + LT + S CC PVDK
Sbjct: 507 VHKSEKEKSHHSKLLVLRSSNHVKTERKKKSLRLTLAGLKSNIIKSSLLHCCQLHFPVDK 328
Query: 303 MH 304
+H
Sbjct: 327 IH 322
>TC225016 similar to UP|Q39835 (Q39835) Extensin, partial (32%)
Length = 973
Score = 28.5 bits (62), Expect = 4.4
Identities = 16/68 (23%), Positives = 28/68 (40%)
Frame = +3
Query: 217 QHSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQLQR 276
+H + T+ PP HL S + H + H HL+ + +TH Q +
Sbjct: 6 RHLLSITTSTLTLPTFTPPHHLLLRSHTTITHHPLLHHLHLSHTI---TTHHHHHHQRRN 176
Query: 277 IILTSINH 284
T+++H
Sbjct: 177 HTTTTLHH 200
>BG839202 similar to GP|19310423|gb At1g10390/F14N23_29 {Arabidopsis
thaliana}, partial (4%)
Length = 685
Score = 28.1 bits (61), Expect = 5.8
Identities = 13/30 (43%), Positives = 18/30 (59%)
Frame = +1
Query: 119 RGTKSSTNMFTKQPISLVITLDSPPPMGST 148
R S+ N F+ +PI + I L +P P GST
Sbjct: 373 RRALSALNRFSDRPIRVAIILFAPKPFGST 462
>TC216994 weakly similar to GB|AAD18096.1|4337175|T31J12 ESTs gb|T20589,
gb|T04648, gb|AA597906, gb|T04111, gb|R84180, gb|R65428,
gb|T44439, gb|T76570, gb|R90004, gb|T45020, gb|T42457,
gb|T20921, gb|AA042762 and gb|AA720210 come from this
gene. {Arabidopsis thaliana;} , partial (73%)
Length = 748
Score = 28.1 bits (61), Expect = 5.8
Identities = 18/53 (33%), Positives = 23/53 (42%)
Frame = +3
Query: 104 STGTKKCYCPFRLRARGTKSSTNMFTKQPISLVITLDSPPPMGSTVHAITSTS 156
+TGTK R +R + S T F + T D PP GS +T TS
Sbjct: 96 TTGTKCAK*SPRSFSRKSPSPTAFFPSRTWRSAATTDKPPSSGSNKRRVTPTS 254
>TC226825 similar to UP|Q9ZW85 (Q9ZW85) 3-isopropylmalate dehydratase, small
subunit, partial (71%)
Length = 1121
Score = 28.1 bits (61), Expect = 5.8
Identities = 17/48 (35%), Positives = 23/48 (47%), Gaps = 3/48 (6%)
Frame = +1
Query: 232 HNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHR---RREGQLQR 276
H PPRHL + R + VRH H + + R RR G+L+R
Sbjct: 337 HRPPRHLRHAFHRTRRDQNQVRHRHRRCQLRLRLLPRARPRRAGRLRR 480
>TC223427
Length = 701
Score = 27.7 bits (60), Expect = 7.6
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Frame = +3
Query: 216 PQHSFRQVSQTV--DTVLHNPPRHLESSSPRRVNHREP 251
PQHSFR S ++ +T L P S+SPR+ R P
Sbjct: 120 PQHSFRNTSHSLHSNTHLRLPTS*TPSASPRKPLSRSP 233
>TC203287 weakly similar to UP|Q41645 (Q41645) Extensin (Fragment), partial
(14%)
Length = 967
Score = 27.7 bits (60), Expect = 7.6
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +1
Query: 232 HNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQLQ 275
H+ +HL PR+ +H + +H H ++ Q H +++ Q Q
Sbjct: 172 HHQDQHLHQHPPRQQHHHQ--QHHHQQQQLLHQQHHHQQQHQRQ 297
>BG790175 similar to PIR|T09854|T098 proline-rich cell wall protein - upland
cotton, partial (21%)
Length = 415
Score = 27.7 bits (60), Expect = 7.6
Identities = 12/44 (27%), Positives = 23/44 (52%)
Frame = +2
Query: 232 HNPPRHLESSSPRRVNHREPVRHGHLTMGVVRQSTHRRREGQLQ 275
H+ +HL PR+ +H + +H H ++ Q H +++ Q Q
Sbjct: 20 HHQDQHLHQHPPRQQHHHQ--QHHHQQQQLLHQQHHHQQQHQRQ 145
>TC233438 similar to UP|Q6XD84 (Q6XD84) SR RNA-binding protein, partial (5%)
Length = 856
Score = 27.7 bits (60), Expect = 7.6
Identities = 18/54 (33%), Positives = 26/54 (47%)
Frame = +2
Query: 200 DSTPTLILSRGAQSVSPQHSFRQVSQTVDTVLHNPPRHLESSSPRRVNHREPVR 253
D +P+ + S+ Q + HS R + H+P L+SS RR R PVR
Sbjct: 434 DLSPSPVRSKRRQLLRSPHSKRSQRR------HSPYTSLDSSRGRRSRSRSPVR 577
Database: GMGI
Posted date: Oct 22, 2004 4:58 PM
Number of letters in database: 37,918,896
Number of sequences in database: 63,676
Lambda K H
0.319 0.133 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,845,195
Number of Sequences: 63676
Number of extensions: 218436
Number of successful extensions: 1317
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 1304
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1312
length of query: 334
length of database: 12,639,632
effective HSP length: 98
effective length of query: 236
effective length of database: 6,399,384
effective search space: 1510254624
effective search space used: 1510254624
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 59 (27.3 bits)
Lotus: description of TM0022.14